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Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations


Simonelli, Luca; Pedotti, Mattia; Bardelli, Marco; Jurt, Simon; Zerbe, Oliver; Varani, Luca (2018). Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations. In: Rockberg, Johan; Nilvebrant, Johan. Epitope Mapping Protocols. Berlin: Springer, 29-51.

Abstract

Identifying an epitope, the region of the antigen in contact with an antibody, is useful in both basic and pharmaceutical research, as well as in vaccine design. Solution NMR spectroscopy is particularly well suited to the residue level characterization of intermolecular interfaces, including antibody-antigen interactions, and thus to epitope identification. Here, we describe the use of NMR for residue level characterization of protein epitopes, focusing on experimental protocols and practical considerations, highlighting advantages and drawbacks of the approach.

Abstract

Identifying an epitope, the region of the antigen in contact with an antibody, is useful in both basic and pharmaceutical research, as well as in vaccine design. Solution NMR spectroscopy is particularly well suited to the residue level characterization of intermolecular interfaces, including antibody-antigen interactions, and thus to epitope identification. Here, we describe the use of NMR for residue level characterization of protein epitopes, focusing on experimental protocols and practical considerations, highlighting advantages and drawbacks of the approach.

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Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:2018
Deposited On:01 Nov 2018 07:49
Last Modified:24 Sep 2019 23:50
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1785
ISSN:1064-3745
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-7841-0_3

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