Header

UZH-Logo

Maintenance Infos

Morphological characterization of basally located uninucleate trophoblast cells as precursors of bovine binucleate trophoblast giant cells


Attiger, Jeannette; Boos, Alois; Klisch, Karl (2018). Morphological characterization of basally located uninucleate trophoblast cells as precursors of bovine binucleate trophoblast giant cells. Cells, Tissues, Organs, 205(3):151-163.

Abstract

Binucleate trophoblast giant cells (TGCs) are one characteristic feature of the ruminant placenta. In cows, the frequency of TGCs remains constant for most of the duration of pregnancy. As TGCs are depleted by their fusion with uterine epithelial cells, they need to be constantly formed. It is still unclear whether they develop from stem cells within the trophectoderm or whether they can arise from any uninucleate trophoblast cell (UTC). Within the latter, generally accepted theory, a basally located uninucleate cell (BUC) without contact to the feto-maternal interface would represent a transient cell between a UTC and a TGC. So far, no evidence for the existence of such transient cells or for the presence of stem cells has been shown. The aim of the present study is to morphologically characterize the early stages of TGC development. Placentomal tissue of 6 pregnant cows from different gestational stages (gestational days 51-214) was examined for BUCs, UTCs, and TGCs either in serial sections (light and transmission electron microscopy, TEM, n = 3), in single sections (TEM, n = 2), or by serial block face-scanning electron microscopy (n = 1). These investigations revealed the occurrence of BUCs, as well as young TGCs showing contact with the basement membrane (BM), but without apical contact to the feto-maternal interface. The study morphologically defines these 2 cell types as early stages of TGC development and shows that binucleation of TGCs can precede detachment from the BM.

Abstract

Binucleate trophoblast giant cells (TGCs) are one characteristic feature of the ruminant placenta. In cows, the frequency of TGCs remains constant for most of the duration of pregnancy. As TGCs are depleted by their fusion with uterine epithelial cells, they need to be constantly formed. It is still unclear whether they develop from stem cells within the trophectoderm or whether they can arise from any uninucleate trophoblast cell (UTC). Within the latter, generally accepted theory, a basally located uninucleate cell (BUC) without contact to the feto-maternal interface would represent a transient cell between a UTC and a TGC. So far, no evidence for the existence of such transient cells or for the presence of stem cells has been shown. The aim of the present study is to morphologically characterize the early stages of TGC development. Placentomal tissue of 6 pregnant cows from different gestational stages (gestational days 51-214) was examined for BUCs, UTCs, and TGCs either in serial sections (light and transmission electron microscopy, TEM, n = 3), in single sections (TEM, n = 2), or by serial block face-scanning electron microscopy (n = 1). These investigations revealed the occurrence of BUCs, as well as young TGCs showing contact with the basement membrane (BM), but without apical contact to the feto-maternal interface. The study morphologically defines these 2 cell types as early stages of TGC development and shows that binucleation of TGCs can precede detachment from the BM.

Statistics

Citations

Altmetrics

Downloads

7 downloads since deposited on 03 Dec 2018
7 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Anatomy, Histology, Cattle; Placenta; Pregnancy; Stem cells; Trophoblast giant cells
Language:English
Date:1 January 2018
Deposited On:03 Dec 2018 09:27
Last Modified:28 Feb 2019 08:28
Publisher:Karger
ISSN:1422-6405
OA Status:Green
Publisher DOI:https://doi.org/10.1159/000489257
PubMed ID:29925059

Download

Download PDF  'Morphological characterization of basally located uninucleate trophoblast cells as precursors of bovine binucleate trophoblast giant cells'.
Preview
Content: Published Version
Language: English
Filetype: PDF
Size: 2MB
View at publisher