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Metagenomic virome sequencing in living donor-recipient kidney transplant pairs revealed JC Polyomavirus transmission


Schreiber, Peter W; Kufner, Verena; Hübel, Kerstin; Schmutz, Stefan; Zagordi, Osvaldo; Kaur, Amandeep; Bayard, Cornelia; Greiner, Michael; Zbinden, Andrea; Capaul, Riccarda; Böni, Jürg; Hirsch, Hans H; Mueller, Thomas F; Mueller, Nicolas J; Trkola, Alexandra; Huber, Michael (2019). Metagenomic virome sequencing in living donor-recipient kidney transplant pairs revealed JC Polyomavirus transmission. Clinical Infectious Diseases, 69(6):987-994.

Abstract

Background Before kidney transplantation, donors and recipients are routinely screened for viral pathogens using specific tests. Little is known about unrecognized viruses of the urinary tract that potentially result in transmission. Using an open metagenomic approach, we aimed to comprehensively assess virus transmission in living donor kidney transplantation. Methods Living kidney donors and their corresponding recipients were enrolled at the time of transplantation. Follow-up study visits for recipients were scheduled 4-6 weeks and 1 year thereafter. At each visit, plasma and urine samples were collected and transplant recipients were evaluated for signs of infection or other transplant-related complications. For metagenomic analysis, samples were enriched for viruses, amplified by anchored random PCR and sequenced using high-throughput metagenomic sequencing. Viruses detected by sequencing were confirmed using real-time PCR. Results We analyzed a total of 30 living kidney donor-recipient pairs with a follow-up of at least one year. In addition to viruses commonly detected during routine post-transplant virus monitoring, metagenomic sequencing detected JC polyomavirus (JCPyV) in urine of seven donors and their corresponding recipients. Phylogenetic analysis confirmed infection with the donor strain in six cases, suggesting transmission from transplant donor to recipient despite recipient seropositivity for JCPyV at the time of transplantation. Conclusions Metagenomic sequencing identified frequent transmission of JCPyV from kidney transplant donors to recipients. Considering the high incidence rate, future studies within larger cohorts are needed to define the relevance of JCPyV infection and the donor's virome for transplant outcome.

Abstract

Background Before kidney transplantation, donors and recipients are routinely screened for viral pathogens using specific tests. Little is known about unrecognized viruses of the urinary tract that potentially result in transmission. Using an open metagenomic approach, we aimed to comprehensively assess virus transmission in living donor kidney transplantation. Methods Living kidney donors and their corresponding recipients were enrolled at the time of transplantation. Follow-up study visits for recipients were scheduled 4-6 weeks and 1 year thereafter. At each visit, plasma and urine samples were collected and transplant recipients were evaluated for signs of infection or other transplant-related complications. For metagenomic analysis, samples were enriched for viruses, amplified by anchored random PCR and sequenced using high-throughput metagenomic sequencing. Viruses detected by sequencing were confirmed using real-time PCR. Results We analyzed a total of 30 living kidney donor-recipient pairs with a follow-up of at least one year. In addition to viruses commonly detected during routine post-transplant virus monitoring, metagenomic sequencing detected JC polyomavirus (JCPyV) in urine of seven donors and their corresponding recipients. Phylogenetic analysis confirmed infection with the donor strain in six cases, suggesting transmission from transplant donor to recipient despite recipient seropositivity for JCPyV at the time of transplantation. Conclusions Metagenomic sequencing identified frequent transmission of JCPyV from kidney transplant donors to recipients. Considering the high incidence rate, future studies within larger cohorts are needed to define the relevance of JCPyV infection and the donor's virome for transplant outcome.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Virology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Nephrology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Infectious Diseases
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:30 August 2019
Deposited On:29 Jan 2019 12:05
Last Modified:06 Oct 2019 05:57
Publisher:Oxford University Press
ISSN:1058-4838
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/cid/ciy1018
PubMed ID:30508036

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