Header

UZH-Logo

Maintenance Infos

Gel-Free Proteomics


Baiwir, Dominique; Nanni, Paolo; Müller, Susanne; Smargiasso, Nicolas; Morsa, Denis; De Pauw, Edwin; Mazzucchelli, Gabriel (2018). Gel-Free Proteomics. In: Martinho de Almeida, Andre. Proteomics in Domestic Animals: from Farm to Systems Biology. Cham, 55-101.

Abstract

Mass spectrometry (MS)-based proteomics is nowadays considered as a mainstream analytical tool for life sciences. This success is mainly to be attributed to the tremendous improvements in the core technology of mass spectrometry coupled with recent developments in bioinformatics and separation sciences. The level of information that is henceforth accessible through MS techniques is broad and encompasses the protein sequence as well as its quantity, modification(s), structure, and implication in macromolecular assemblies. The current chapter focuses on the existing gel-free MS-based quantitative approaches that are applicable in the field of animal sciences and summarizes their principal features together with their respective advantages and drawbacks. In this context, we will discuss both the label-free and label-based relative quantitation strategies as well as the existing alternatives for absolute quantitation. The specific case of posttranslational modifications, whose extensive characterization is becoming a crucial topic in MS-based proteome studies, is next addressed under the quantitative scope of phosphorylation and glycosylation. Lastly, other promising nonmainstream techniques are presented, emphasizing their potent use to solve major proteomics challenges of tomorrow.

Abstract

Mass spectrometry (MS)-based proteomics is nowadays considered as a mainstream analytical tool for life sciences. This success is mainly to be attributed to the tremendous improvements in the core technology of mass spectrometry coupled with recent developments in bioinformatics and separation sciences. The level of information that is henceforth accessible through MS techniques is broad and encompasses the protein sequence as well as its quantity, modification(s), structure, and implication in macromolecular assemblies. The current chapter focuses on the existing gel-free MS-based quantitative approaches that are applicable in the field of animal sciences and summarizes their principal features together with their respective advantages and drawbacks. In this context, we will discuss both the label-free and label-based relative quantitation strategies as well as the existing alternatives for absolute quantitation. The specific case of posttranslational modifications, whose extensive characterization is becoming a crucial topic in MS-based proteome studies, is next addressed under the quantitative scope of phosphorylation and glycosylation. Lastly, other promising nonmainstream techniques are presented, emphasizing their potent use to solve major proteomics challenges of tomorrow.

Statistics

Citations

Dimensions.ai Metrics

Altmetrics

Additional indexing

Item Type:Book Section, not_refereed, original work
Communities & Collections:04 Faculty of Medicine > Functional Genomics Center Zurich
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2 February 2018
Deposited On:22 Feb 2019 09:53
Last Modified:25 Sep 2019 00:08
ISBN:978 3 319 69681 2
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-3-319-69682-9_5

Download

Full text not available from this repository.
View at publisher

Get full-text in a library