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Profiling of boar taint by nontargeted metabolomics


Olson, Malin Emelie Maria. Profiling of boar taint by nontargeted metabolomics. 2013, University of Zurich, Vetsuisse Faculty.

Abstract

In many countries, male piglets are castrated shortly after birth to avoid the production of meat with an unpleasant smell and flavour known as boar taint. Extensive research has been carried out during the last 40 years to delineate compounds that are responsible for this problem. The most frequently candidates are androstenone, skatole and indole. However, other factors must be involved in causing boar taint, since a significant proportion of tainted pigs have unchanged levels of these three compounds. The aim of this thesis was to establish the conditions for a non-targeted metabolomics study and thereby identify new potential biomarkers that correlate with the appearance of boar taint. The adipose tissue of 16 nontainted and 17 strongly tainted pigs, selected by an earlier sensory panel analysis, was homogenized with methanol. After solid-phase extraction, the samples were analyzed by liquid chromatography coupled to a time-of-flight mass spectrometer using a nanoUPLC®- ESI-QTOF-HDMS™ system. By monitoring about 20’000 different masses with an accuracy of around 5 ppm, we found a metabolic pattern that is characteristic for the appearance of boar taint. A set of 16 masses can discriminate between tainted and non-tainted carcasses with a mean predictive accuracy of 90%. These results will be used to further develop a reliable test for the rapid detection of boar-tainted meat.

Abstract

In many countries, male piglets are castrated shortly after birth to avoid the production of meat with an unpleasant smell and flavour known as boar taint. Extensive research has been carried out during the last 40 years to delineate compounds that are responsible for this problem. The most frequently candidates are androstenone, skatole and indole. However, other factors must be involved in causing boar taint, since a significant proportion of tainted pigs have unchanged levels of these three compounds. The aim of this thesis was to establish the conditions for a non-targeted metabolomics study and thereby identify new potential biomarkers that correlate with the appearance of boar taint. The adipose tissue of 16 nontainted and 17 strongly tainted pigs, selected by an earlier sensory panel analysis, was homogenized with methanol. After solid-phase extraction, the samples were analyzed by liquid chromatography coupled to a time-of-flight mass spectrometer using a nanoUPLC®- ESI-QTOF-HDMS™ system. By monitoring about 20’000 different masses with an accuracy of around 5 ppm, we found a metabolic pattern that is characteristic for the appearance of boar taint. A set of 16 masses can discriminate between tainted and non-tainted carcasses with a mean predictive accuracy of 90%. These results will be used to further develop a reliable test for the rapid detection of boar-tainted meat.

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Additional indexing

Item Type:Dissertation (monographical)
Referees:Naegeli Hanspeter, Torgerson Paul R
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pharmacology and Toxicology
05 Vetsuisse Faculty > Chair in Veterinary Epidemiology
UZH Dissertations
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Boar taint, Metabolomics, Adipose tissue, nanoUPLC, Mass Spectrometry
Language:English
Place of Publication:Zürich
Date:2013
Deposited On:02 May 2019 14:18
Last Modified:25 Sep 2019 00:13
Number of Pages:59
OA Status:Green
Related URLs:https://www.recherche-portal.ch/primo-explore/fulldisplay?docid=ebi01_prod009923335&context=L&vid=ZAD&search_scope=default_scope&tab=default_tab&lang=de_DE (Library Catalogue)

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