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Antimicrobial resistance, multilocus sequence types and virulence profiles of ESBL producing and non-ESBL producing uropathogenic Escherichia coli isolated from cats and dogs in Switzerland


Zogg, Anna Lena. Antimicrobial resistance, multilocus sequence types and virulence profiles of ESBL producing and non-ESBL producing uropathogenic Escherichia coli isolated from cats and dogs in Switzerland. 2018, University of Zurich, Vetsuisse Faculty.

Abstract

Unter 64 uropathogenen Escherichia coli (UPEC), welche von 13 Katzen und 51 Hunden isoliert wurden, fanden sich 35 extended-spectrum -Lactamase (ESBL) produzierende UPEC, die restlichen 29 waren nicht-ESBL Bildner. 46 (71.9%) Isolate waren multiresistent (MDR). Innerhalb der ESBL Bildner wurden folgende blaESBL Gene identifiziert: blaCTX-M-15 (n=17/48.6%), blaCTX-M-1 (n=10/28.6%), blaCTX-M-55 (n=4/11.4%), blaCTX-M-14 (n=3/8.6%) und blaCTX-M-27 (n=1/2.9%). Die Plasmid-mediierten Gene der Fluorchinolonresistenz aac(6')-Ib- cr und qnrB sowie das Azithromycinresistenzgen mph(A) wurden in 17 (26.6%) Isolaten, beziehungsweise in einem (1.6%) und in 13 (20.3%) gefunden. Die am häufigsten vorkommenden Phylogruppen waren Gruppe C (n=19) und B2 (n=15). Es wurden 26 verschiedene Sequenz-Typen (ST) identifiziert, wobei zwei davon neu waren. Die häufigsten ST waren ST410 (n=16/25%), ST131 und ST73 (beide n=5/7.8%) und ST361 (n=4/6.3%). Zehn (15.6%) der ST stehen in Verbindung zu Harnwegsinfektionen (HWI) beim Menschen. Von den sieben Virulenz-assozierten Genen war fyuA das am häufigsten vorkommende. Die Gesamtzahl an Virulenzfaktoren (VF) war am höchsten bei Isolaten, die zur Phylogruppe B2 (mittlere Gesamtzahlrate 6, Hauptrate 5.5, Streubreite 3-7) gehören, am niedrigsten für Isolate der Phylogruppe C (0/ 0.5/ 0-3). Der häufigste ST in dieser Studie, ST410, wies die geringste Gesamtzahl an VF (0/ 0.3/ 0-2) auf.

Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n=17/48.6% of the blaESBLs), blaCTX-M-1 (n=10/28.6%), blaCTX-M- 55 (n=4/11.4%), blaCTX-M-14 (n=3/8.6%), and blaCTX-M-27 (n=1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26.6% of all isolates), one (1.6%) and in 13 (20.3%) respectively. The most frequent phylogenetic groups were C (n=19) and B2 (n=15). Twenty-six different sequence types (STs) were identified, with two being novel. The most frequent STs were ST410 (n=16/25%), ST131, and ST73 (both n=5/7.8%), and ST361 (n=4/6.3%). Ten (15.6%) of the STs have been associated with urinary tract infection (UTI) in humans, suggesting zoonotic potential. Among seven virulence-associated genes, fyuA was the most prevalent. The overall aggregate virulence factor (VF) score was highest for isolates belonging to phylogenetic group B2 (median aggregate VF score 6, mean score 5,5, range 3- 7), and lowest for isolates belonging to phylogenetic group C (0/ 0.5/0-3). The most frequent ST in this study, ST410, harboured the lowest number of VF (0/0,3/0-2).

Abstract

Unter 64 uropathogenen Escherichia coli (UPEC), welche von 13 Katzen und 51 Hunden isoliert wurden, fanden sich 35 extended-spectrum -Lactamase (ESBL) produzierende UPEC, die restlichen 29 waren nicht-ESBL Bildner. 46 (71.9%) Isolate waren multiresistent (MDR). Innerhalb der ESBL Bildner wurden folgende blaESBL Gene identifiziert: blaCTX-M-15 (n=17/48.6%), blaCTX-M-1 (n=10/28.6%), blaCTX-M-55 (n=4/11.4%), blaCTX-M-14 (n=3/8.6%) und blaCTX-M-27 (n=1/2.9%). Die Plasmid-mediierten Gene der Fluorchinolonresistenz aac(6')-Ib- cr und qnrB sowie das Azithromycinresistenzgen mph(A) wurden in 17 (26.6%) Isolaten, beziehungsweise in einem (1.6%) und in 13 (20.3%) gefunden. Die am häufigsten vorkommenden Phylogruppen waren Gruppe C (n=19) und B2 (n=15). Es wurden 26 verschiedene Sequenz-Typen (ST) identifiziert, wobei zwei davon neu waren. Die häufigsten ST waren ST410 (n=16/25%), ST131 und ST73 (beide n=5/7.8%) und ST361 (n=4/6.3%). Zehn (15.6%) der ST stehen in Verbindung zu Harnwegsinfektionen (HWI) beim Menschen. Von den sieben Virulenz-assozierten Genen war fyuA das am häufigsten vorkommende. Die Gesamtzahl an Virulenzfaktoren (VF) war am höchsten bei Isolaten, die zur Phylogruppe B2 (mittlere Gesamtzahlrate 6, Hauptrate 5.5, Streubreite 3-7) gehören, am niedrigsten für Isolate der Phylogruppe C (0/ 0.5/ 0-3). Der häufigste ST in dieser Studie, ST410, wies die geringste Gesamtzahl an VF (0/ 0.3/ 0-2) auf.

Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n=17/48.6% of the blaESBLs), blaCTX-M-1 (n=10/28.6%), blaCTX-M- 55 (n=4/11.4%), blaCTX-M-14 (n=3/8.6%), and blaCTX-M-27 (n=1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26.6% of all isolates), one (1.6%) and in 13 (20.3%) respectively. The most frequent phylogenetic groups were C (n=19) and B2 (n=15). Twenty-six different sequence types (STs) were identified, with two being novel. The most frequent STs were ST410 (n=16/25%), ST131, and ST73 (both n=5/7.8%), and ST361 (n=4/6.3%). Ten (15.6%) of the STs have been associated with urinary tract infection (UTI) in humans, suggesting zoonotic potential. Among seven virulence-associated genes, fyuA was the most prevalent. The overall aggregate virulence factor (VF) score was highest for isolates belonging to phylogenetic group B2 (median aggregate VF score 6, mean score 5,5, range 3- 7), and lowest for isolates belonging to phylogenetic group C (0/ 0.5/0-3). The most frequent ST in this study, ST410, harboured the lowest number of VF (0/0,3/0-2).

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Additional indexing

Other titles:Antibiotikaresistenz, MLST Sequenztypen und Virulenzprofile von ESBL-bildenden und nicht ESLB-bildenden uropathogenen Escherichia coli Isolaten von Katzen und Hunden in der Schweiz
Item Type:Dissertation (monographical)
Referees:Stephan Roger
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
UZH Dissertations
Dewey Decimal Classification:630 Agriculture
Uncontrolled Keywords:uropathogenic, Escherichia coli, cats, dogs, MLST, virulence
Language:English
Place of Publication:Zürich
Date:2018
Deposited On:24 Jan 2019 16:23
Last Modified:15 Apr 2021 15:02
Number of Pages:22
OA Status:Green
Related URLs:https://www.zora.uzh.ch/id/eprint/167650/

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