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Influence of feeding and UVB exposition on the absorption mechanisms of calcium in the gastrointestinal tract of veiled chameleons (Chamaeleo calyptratus)


Haxhiu, Driton. Influence of feeding and UVB exposition on the absorption mechanisms of calcium in the gastrointestinal tract of veiled chameleons (Chamaeleo calyptratus). 2014, University of Zurich, Vetsuisse Faculty.

Abstract

The aim of this study was to investigate the influence of feeding and UVB exposition on the occurrence and distribution patterns of vitamin D receptors (VDR) and calbindin D28k (Cb-D28k) in the gastrointestinal tract of veiled chameleons. Thus, 56 hatchlings were divided into 6 treatment groups: UV (with UVB exposure); No (no supplements); CaAUV (with calcium (Ca), vitamin A (vitA), UVB exposure); CaA (Ca, vitA); CaADUV (Ca, vitA, vitD, UVB exposure); CaAD (Ca, vitA, vitD) and reared for 6 months on locust-based diets. Finally, tissue sections of stomach, duodenum, ileum and colon were stained immunohistochemically (IHC) against Cb- D28k or VDR and assessed semi-quantitatively. VDR stained stronger in the luminal epithelium of duodenum than ileum, with more intense signals localized at the base of the villi when compared with the tip. Cb-D28k was mostly targeted to the luminal epithelium of the duodenum. In CaADUV and CaAD (groups with all supplements) Cb-D28k staining was stronger than in UV group. The important role of duodenum in the active transcellular absorption of Ca in veiled chameleons is suggested. Whereas the expression of Cb-D28k seems to be regulated by dietary supplementation with vit. D and A, the expression of VDR appears higher in animals not supplemented with Ca or vit D and A. The latter may be due to the decreased Ca concentrations, causing mobilization of vitamin D in the skin of animals exposed to UVB only.

Abstract

The aim of this study was to investigate the influence of feeding and UVB exposition on the occurrence and distribution patterns of vitamin D receptors (VDR) and calbindin D28k (Cb-D28k) in the gastrointestinal tract of veiled chameleons. Thus, 56 hatchlings were divided into 6 treatment groups: UV (with UVB exposure); No (no supplements); CaAUV (with calcium (Ca), vitamin A (vitA), UVB exposure); CaA (Ca, vitA); CaADUV (Ca, vitA, vitD, UVB exposure); CaAD (Ca, vitA, vitD) and reared for 6 months on locust-based diets. Finally, tissue sections of stomach, duodenum, ileum and colon were stained immunohistochemically (IHC) against Cb- D28k or VDR and assessed semi-quantitatively. VDR stained stronger in the luminal epithelium of duodenum than ileum, with more intense signals localized at the base of the villi when compared with the tip. Cb-D28k was mostly targeted to the luminal epithelium of the duodenum. In CaADUV and CaAD (groups with all supplements) Cb-D28k staining was stronger than in UV group. The important role of duodenum in the active transcellular absorption of Ca in veiled chameleons is suggested. Whereas the expression of Cb-D28k seems to be regulated by dietary supplementation with vit. D and A, the expression of VDR appears higher in animals not supplemented with Ca or vit D and A. The latter may be due to the decreased Ca concentrations, causing mobilization of vitamin D in the skin of animals exposed to UVB only.

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Additional indexing

Item Type:Dissertation (monographical)
Referees:Liesegang Annette
Communities & Collections:05 Vetsuisse Faculty > Institute of Animal Nutrition
UZH Dissertations
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Uncontrolled Keywords:veiled chameleon, calcium regulation, gastrointestinal tract, nutrition
Language:English
Place of Publication:Zürich
Date:2014
Deposited On:29 Apr 2019 15:46
Last Modified:28 Oct 2019 08:28
Number of Pages:21
OA Status:Green
Related URLs:https://www.recherche-portal.ch/primo-explore/fulldisplay?docid=ebi01_prod010260545&context=L&vid=ZAD&search_scope=default_scope&tab=default_tab&lang=de_DE (Library Catalogue)

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