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Telomere length and redox balance in master endurance runners: The role of nitric oxide


Sousa, Caio V; Aguiar, Samuel S; Santos, Patrick A; Barbosa, Lucas P; Knechtle, Beat; Nikolaidis, Pantelis T; Deus, Lysleine A; Sales, Marcelo M; Rosa, Erica C C C; Rosa, Thiago S; Lewis, John E; Andrade, Rosângela V; Simões, Herbert G (2019). Telomere length and redox balance in master endurance runners: The role of nitric oxide. Experimental Gerontology, 117:113-118.

Abstract

Leukocyte telomere length (LTL), a biological marker of aging that is associated with age-related diseases, is longer in master endurance runners (ER) than age-matched controls, but the underlying mechanisms are poorly investigated. The LTL, nitric oxide (NO), and redox balance of ER master runners were analyzed and compared to untrained middle-aged and young adults. We hypothesized that NO and redox balance at baseline would be related to longer LTL in ER athletes. Participants (n = 38) were long-term ER runners (n = 10; 51.6 ± 5.2 yrs.; 28.4 ± 9.4 yrs. of experience) and untrained age-matched (n = 17; 46.6 ± 7.1 yrs) and young controls (n = 11; 21.8 ± 4.0 yrs). Volunteers were assessed for anamnesis, anthropometrics, and blood sampling. Measurements of pro-and anti-oxidant status and DNA extraction were performed using commercial kits. Relative LTL was determined with qPCR analyses (T/S). While the middle-aged controls had shorter LTL than the young group, no difference was observed between ER athletes and young participants. A large effect size between the LTL of ER athletes and middle-aged controls (d = 0.85) was also observed. The ER athletes and untrained young group had better redox balance according to antioxidant/pro-oxidant ratios compared to middle-aged untrained participants, which also had lower values for redox parameters (TEAC/TBARS, SOD/TBARS, and CAT/TBARS; all p < 0.05). Furthermore, the NO level of ER athletes (175.2 ± 31.9 μM) was higher (p < 0.05) than middle-aged controls (67.2 ± 23.3 μM) and young participants (129.2 ± 17.3 μM), with a significant correlation with LTL (r = 0.766; p = 0.02). In conclusion, ER runners have longer LTL than age-matched controls, which in turn may be related to better NO bioavailability and redox balance status.

Abstract

Leukocyte telomere length (LTL), a biological marker of aging that is associated with age-related diseases, is longer in master endurance runners (ER) than age-matched controls, but the underlying mechanisms are poorly investigated. The LTL, nitric oxide (NO), and redox balance of ER master runners were analyzed and compared to untrained middle-aged and young adults. We hypothesized that NO and redox balance at baseline would be related to longer LTL in ER athletes. Participants (n = 38) were long-term ER runners (n = 10; 51.6 ± 5.2 yrs.; 28.4 ± 9.4 yrs. of experience) and untrained age-matched (n = 17; 46.6 ± 7.1 yrs) and young controls (n = 11; 21.8 ± 4.0 yrs). Volunteers were assessed for anamnesis, anthropometrics, and blood sampling. Measurements of pro-and anti-oxidant status and DNA extraction were performed using commercial kits. Relative LTL was determined with qPCR analyses (T/S). While the middle-aged controls had shorter LTL than the young group, no difference was observed between ER athletes and young participants. A large effect size between the LTL of ER athletes and middle-aged controls (d = 0.85) was also observed. The ER athletes and untrained young group had better redox balance according to antioxidant/pro-oxidant ratios compared to middle-aged untrained participants, which also had lower values for redox parameters (TEAC/TBARS, SOD/TBARS, and CAT/TBARS; all p < 0.05). Furthermore, the NO level of ER athletes (175.2 ± 31.9 μM) was higher (p < 0.05) than middle-aged controls (67.2 ± 23.3 μM) and young participants (129.2 ± 17.3 μM), with a significant correlation with LTL (r = 0.766; p = 0.02). In conclusion, ER runners have longer LTL than age-matched controls, which in turn may be related to better NO bioavailability and redox balance status.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of General Practice
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Aging
Life Sciences > Molecular Biology
Life Sciences > Genetics
Life Sciences > Endocrinology
Life Sciences > Cell Biology
Language:English
Date:1 March 2019
Deposited On:14 Mar 2019 16:49
Last Modified:15 Apr 2020 23:07
Publisher:Elsevier
ISSN:0531-5565
OA Status:Green
Publisher DOI:https://doi.org/10.1016/j.exger.2018.11.018
PubMed ID:30481549

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