Header

UZH-Logo

Maintenance Infos

In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption


Anderson, Annette Carola; Rothballer, Michael; Altenburger, Markus Jörg; Woelber, Johan Peter; Karygianni, Lamprini; Lagkouvardos, Ilias; Hellwig, Elmar; Al-Ahmad, Ali (2018). In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption. Scientific Reports, 8(1):14202.

Abstract

Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption. Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm. Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples. After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase. The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region). In addition, roughness of the enamel surface was measured with laser scanning microscopy. The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness. It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis. Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface. The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.

Abstract

Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption. Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm. Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples. After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase. The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region). In addition, roughness of the enamel surface was measured with laser scanning microscopy. The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness. It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis. Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface. The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.

Statistics

Citations

Altmetrics

Downloads

15 downloads since deposited on 06 Mar 2019
15 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:21 September 2018
Deposited On:06 Mar 2019 15:28
Last Modified:06 Mar 2019 15:28
Publisher:Nature Publishing Group
ISSN:2045-2322
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1038/s41598-018-32544-6
PubMed ID:30242260

Download

Download PDF  'In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 2MB
View at publisher
Download PDF  'In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption'.
Preview
Content: Published Version
Filetype: PDF
Size: 2MB
Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)