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Evaluation of the bioactivity of fluoride-enriched mineral trioxide aggregate on osteoblasts

Proksch, S; Brossart, J; Vach, K; Hellwig, E; Altenburger, M J; Karygianni, L (2018). Evaluation of the bioactivity of fluoride-enriched mineral trioxide aggregate on osteoblasts. International Endodontic Journal, 51(8):912-923.

Abstract

AIM
To investigate whether a combination of mineral trioxide aggregate (MTA) and fluoride compounds affects bone cells.

METHODOLOGY
Mineral trioxide aggregate (MTA) discs (ProRoot , Dentsply Sirona, Ballaigues, Switzerland) with and without the addition of 0.1%, 0.25% and 0.5% sodium fluoride were characterized for their surface roughness by laser scanning microscopy and for the adhesion of human alveolar osteoblasts by scanning electron microscopy. Using eluates from fluoride-enriched MTA discs, the cell proliferation was measured by monitoring the DNA incorporation of 5-bromo-2'-deoxyuridine. Further, gene expression was evaluated by qPCR arrays, extracellular matrix mineralization was quantified by absorption measurement of Alizarin red stains, and effects were calculated with repeated measures analysis and post hoc P-value adjustment.

RESULTS
Irrespective of fluoride addition, cell adhesion was similar on MTA discs, of which the surface roughness was comparable. Control osteoblasts had a curvilinear proliferation pattern peaking at d5, which was levelled out by incubation with MTA. The addition of fluoride partly restored the MTA-related reduction in the cellular proliferation rate in a dose-dependent manner. At the mRNA level, both fluoride and MTA modulated a number of genes involved in osteogenesis, bone mineral metabolism and extracellular matrix formation. Although MTA significantly impaired extracellular matrix mineralization, the addition of fluoride supported the formation of mineralized nodules in a dose-dependent manner.

CONCLUSION
The addition of fluoride modulated the biocompatibility of MTA in terms of supporting bone cell proliferation and hard tissue formation. Hence, fluoride enrichment is a trend-setting advancement for MTA-based endodontic therapies.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Health Sciences > General Dentistry
Language:English
Date:August 2018
Deposited On:06 Mar 2019 15:01
Last Modified:21 Oct 2024 01:36
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:0143-2885
Additional Information:This is the peer reviewed version of the following article: International Endodontic Journal, 51, 912–923, 2018. , which has been published in final form at https://doi.org/10.1111/iej.12905. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. (http://www.wileyauthors.com/self-archiving)
OA Status:Green
Publisher DOI:https://doi.org/10.1111/iej.12905
PubMed ID:29397012
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