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Uniform light delivery in volumetric optoacoustic tomography


Mc Larney, Ben; Rebling, Johannes; Chen, Zhenyue; Deán-Ben, Xosé Luis; Gottschalk, Sven; Razansky, Daniel (2019). Uniform light delivery in volumetric optoacoustic tomography. Journal of Biophotonics, 12(6):e201800387.

Abstract

Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non-uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimised illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom-made optical fiber illumination. The custom-designed sample chamber ensures convenient access to the imaged object along with accurate positioning of the sample and a matrix array ultrasound transducer used for collection of the volumetric image data. Raytracing is employed to optimize the positioning of the individual fibers in the chamber. Homogeneity of the generated light excitation field was confirmed in tissue-mimicking agar spheres. Applicability of the system to image entire mouse organs ex vivo has been showcased. The new approach showed a clear advantage over conventional, single-sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality. This article is protected by copyright.

Abstract

Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non-uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimised illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom-made optical fiber illumination. The custom-designed sample chamber ensures convenient access to the imaged object along with accurate positioning of the sample and a matrix array ultrasound transducer used for collection of the volumetric image data. Raytracing is employed to optimize the positioning of the individual fibers in the chamber. Homogeneity of the generated light excitation field was confirmed in tissue-mimicking agar spheres. Applicability of the system to image entire mouse organs ex vivo has been showcased. The new approach showed a clear advantage over conventional, single-sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality. This article is protected by copyright.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology

04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Scopus Subject Areas:Physical Sciences > General Chemistry
Physical Sciences > General Materials Science
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Physical Sciences > General Engineering
Physical Sciences > General Physics and Astronomy
Language:English
Date:1 June 2019
Deposited On:15 Mar 2019 12:23
Last Modified:29 Jul 2020 10:15
Publisher:Wiley-VCH Verlag
ISSN:1864-063X
Additional Information:For accepted manuscripts: This is the peer reviewed version of the following article: Volume12, Issue6, June 2019, e201800387, which has been published in final form at https://doi.org/10.1002/jbio.201800387. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
OA Status:Green
Publisher DOI:https://doi.org/10.1002/jbio.201800387
PubMed ID:30701679

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