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Uterine fluid proteome changes during diapause and resumption of embryo development in roe deer


van der Weijden, Vera Anna; Bick, Jochen; Bauersachs, Stefan; Arnold, Georg J; Fröhlich, Thomas; Drews, Barbara; Ulbrich, Susanne E (2019). Uterine fluid proteome changes during diapause and resumption of embryo development in roe deer. Reproduction, 158(1):13-24.

Abstract

The uterine microenvironment during pre-implantation presents a pro-survival milieu, and is essential for embryo elongation in ruminants. The European roe deer (Careolus capreolus) pre-implantation embryo development is characterised by a 4-month period of reduced development, embryonic diapause, after which the embryo rapidly elongates and implants. We investigated the uterine fluid proteome by label-free liquid chromatography tandem mass spectrometry at four defined stages covering the phase of reduced developmental pace (early diapause, mid diapause, and late diapause) and embryo elongation. We hypothesised that embryo development during diapause is halted by the lack of signals that support progression past the blastocyst stage. Three clusters of differentially abundant proteins were identified by a self-organizing tree algorithm: 1) gradual reduction over development; 2) stable abundance during diapause, followed by a sharp rise at elongation; and 3) gradual increase over development. Proteins in the different clusters were subjected to Gene Ontology analysis. ‘Cellular detoxification’ in cluster 1 was represented by alcohol dehydrogenase, glutathione S-transferase, and peroxiredoxin-2. ATP-citrate synthase, nucleolin, lamin A/C, and purine phosphorylase as cell proliferation regulators were found in cluster 2, and ‘cortical cytoskeleton’, ‘regulation of substrate adhesion-dependent cell spreading’, and ‘melanosome’ were present in cluster 3. Cell cycle promoters were higher abundant at elongation than during diapause, and polyamines presence indicates their role in diapause regulation. This study provides a comprehensive overview of proteins in the roe deer uterine fluid during diapause, and forms a basis for studies aiming at understanding the impact of the lack of cell cycle promoters during diapause.

Abstract

The uterine microenvironment during pre-implantation presents a pro-survival milieu, and is essential for embryo elongation in ruminants. The European roe deer (Careolus capreolus) pre-implantation embryo development is characterised by a 4-month period of reduced development, embryonic diapause, after which the embryo rapidly elongates and implants. We investigated the uterine fluid proteome by label-free liquid chromatography tandem mass spectrometry at four defined stages covering the phase of reduced developmental pace (early diapause, mid diapause, and late diapause) and embryo elongation. We hypothesised that embryo development during diapause is halted by the lack of signals that support progression past the blastocyst stage. Three clusters of differentially abundant proteins were identified by a self-organizing tree algorithm: 1) gradual reduction over development; 2) stable abundance during diapause, followed by a sharp rise at elongation; and 3) gradual increase over development. Proteins in the different clusters were subjected to Gene Ontology analysis. ‘Cellular detoxification’ in cluster 1 was represented by alcohol dehydrogenase, glutathione S-transferase, and peroxiredoxin-2. ATP-citrate synthase, nucleolin, lamin A/C, and purine phosphorylase as cell proliferation regulators were found in cluster 2, and ‘cortical cytoskeleton’, ‘regulation of substrate adhesion-dependent cell spreading’, and ‘melanosome’ were present in cluster 3. Cell cycle promoters were higher abundant at elongation than during diapause, and polyamines presence indicates their role in diapause regulation. This study provides a comprehensive overview of proteins in the roe deer uterine fluid during diapause, and forms a basis for studies aiming at understanding the impact of the lack of cell cycle promoters during diapause.

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Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Uncontrolled Keywords:Obstetrics and Gynaecology, Cell Biology, Embryology, Endocrinology, Reproductive Medicine
Language:English
Date:1 July 2019
Deposited On:28 Apr 2019 13:57
Last Modified:25 Sep 2019 00:33
Publisher:BioScientifica Ltd.
ISSN:1470-1626
OA Status:Green
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1530/rep-19-0022
PubMed ID:30933930

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Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)