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Tissue Clearing and Light Sheet Microscopy: Imaging the Unsectioned Adult Zebra Finch Brain at Cellular Resolution

Rocha, Mariana Diales; Düring, Daniel Normen; Bethge, Philipp; Voigt, Fabian F; Hildebrand, Staffan; Helmchen, Fritjof; Pfeifer, Alexander; Hahnloser, Richard H R; Gahr, Manfred (2019). Tissue Clearing and Light Sheet Microscopy: Imaging the Unsectioned Adult Zebra Finch Brain at Cellular Resolution. Frontiers in Neuroanatomy, 13:13.

Abstract

The inherent complexity of brain tissue, with brain cells intertwining locally and projecting to distant regions, has made three-dimensional visualization of intact brains a highly desirable but challenging task in neuroscience. The natural opaqueness of tissue has traditionally limited researchers to techniques short of single cell resolution such as computer tomography or magnetic resonance imaging. By contrast, techniques with single-cell resolution required mechanical slicing into thin sections, which entails tissue distortions that severely hinder accurate reconstruction of large volumes. Recent developments in tissue clearing and light sheet microscopy have made it possible to investigate large volumes at micrometer resolution. The value of tissue clearing has been shown in a variety of tissue types and animal models. However, its potential for examining the songbird brain remains unexplored. Songbirds are an established model system for the study of vocal learning and sensorimotor control. They share with humans the capacity to adapt vocalizations based on auditory input. Song learning and production are controlled in songbirds by the song system, which forms a network of interconnected discrete brain nuclei. Here, we use the CUBIC and iDISCO+ protocols for clearing adult songbird brain tissue. Combined with light sheet imaging, we show the potential of tissue clearing for the investigation of connectivity between song nuclei, as well as for neuroanatomy and brain vasculature studies.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Neuroinformatics
04 Faculty of Medicine > Brain Research Institute
Special Collections > Centers of Competence > Center for the Interdisciplinary Study of Language Evolution
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Health Sciences > Anatomy
Life Sciences > Neuroscience (miscellaneous)
Life Sciences > Cellular and Molecular Neuroscience
Language:English
Date:14 February 2019
Deposited On:16 May 2019 11:26
Last Modified:21 Oct 2024 01:38
Publisher:Frontiers Research Foundation
ISSN:1662-5129
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.3389/fnana.2019.00013
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  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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