The purpose of this study was to compare internal and external load measures during two regimens (6 x 3' and 3 x 6') of a 5 vs. 5 format of play. Moreover, within-regimen changes (between sets) were also tested. Ten amateur soccer players (age: 19.8 ± 1.6 years; experience: 8.3 ± 2.1 years; height: 177.4 ± 3.8 cm; weight: 71.7 ± 4.2 kg) participated in the experiment. Internal load was measured using the CR-10 scale as the rated of perceived exertion (RPE) scale and a heart rate (HR) monitor. The measurements of total (TD), running (RD) and sprinting (SD) distances were also collected using a 10-Hz validated and reliable GPS. Comparisons between regimens revealed that the 3 x 6' regimen was significantly more intense in terms of RPE than the 6 x 3' regimen (p = 0.028; d = 0.351), although no significant differences were found in HR. Significantly greater averages of TD (p = 0.000; d = 0.871) and RD (p = 0.004; d = 0.491) were found in the 6 x 3' regimen. In both regimens, the RPE was significantly lower during the first set than in the remaining sets. On the other hand, the TD was significantly shorter in the last sets than in the earlier. In summary, the present study suggests that shorter sets may be beneficial for maintaining higher internal and external load intensities during 5 vs. 5 formats, and that a drop-in performance may occur throughout the sets in both regimens.