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Smart Toolkit for Fluorescence Tomography: simulation, reconstruction, and validation


Ren, Wuwei; Isler, Helene; Wolf, Martin; Ripoll, Jorge; Rudin, Markus (2019). Smart Toolkit for Fluorescence Tomography: simulation, reconstruction, and validation. IEEE Transactions on Bio-Medical Engineering:Epub ahead of print.

Abstract

Abstract:
Objective: Fluorescence molecular tomography (FMT) can provide valuable molecular information by mapping the bio-distribution of fluorescent reporter molecules in the intact organism. Various prototype FMT systems have been introduced during the past decade. However, none of them has evolved as a standard tool for routine biomedical research. The goal of this work is to develop a software package that can automate the complete FMT reconstruction procedure. Methods: We present Smart Toolkit for Fluorescence Tomography (STIFT), a comprehensive platform comprising three major protocols: i) virtual FMT, i.e., forward modelling and reconstruction of simulated data, ii) control of actual FMT data acquisition, and iii) reconstruction of experimental FMT data. Results: Both simulation and phantom experiments have shown robust reconstruction results for homogeneous and heterogeneous tissue-mimicking phantoms containing fluorescent inclusions. Conclusion: STIFT can be used for optimization of FMT experiments, in particular for optimizing illumination patterns. Significance: This work facilitates FMT experiments by bridging the gaps between simulation, actual experiments, and data reconstruction.

Abstract

Abstract:
Objective: Fluorescence molecular tomography (FMT) can provide valuable molecular information by mapping the bio-distribution of fluorescent reporter molecules in the intact organism. Various prototype FMT systems have been introduced during the past decade. However, none of them has evolved as a standard tool for routine biomedical research. The goal of this work is to develop a software package that can automate the complete FMT reconstruction procedure. Methods: We present Smart Toolkit for Fluorescence Tomography (STIFT), a comprehensive platform comprising three major protocols: i) virtual FMT, i.e., forward modelling and reconstruction of simulated data, ii) control of actual FMT data acquisition, and iii) reconstruction of experimental FMT data. Results: Both simulation and phantom experiments have shown robust reconstruction results for homogeneous and heterogeneous tissue-mimicking phantoms containing fluorescent inclusions. Conclusion: STIFT can be used for optimization of FMT experiments, in particular for optimizing illumination patterns. Significance: This work facilitates FMT experiments by bridging the gaps between simulation, actual experiments, and data reconstruction.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Neonatology
Dewey Decimal Classification:610 Medicine & health
Uncontrolled Keywords:Biomedical Engineering
Language:English
Date:1 January 2019
Deposited On:20 Jun 2019 14:42
Last Modified:20 Jun 2019 14:42
Publisher:Institute of Electrical and Electronics Engineers
ISSN:0018-9294
OA Status:Closed
Publisher DOI:https://doi.org/10.1109/tbme.2019.2907460
Official URL:https://ieeexplore.ieee.org/document/8688443
PubMed ID:30990170
Project Information:
  • : FunderSNSF
  • : Grant ID20B1-1_178262
  • : Project TitleKaleiBox: a portable device for multifunctional fluorescence tomography

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