Abstract
The procedure for the determination of Di‐n‐butyl phthalate (DnBP) and diisobutyl phthalate (DiBP) metabolites corresponds to the HPLC–MS/MS method for the determination of the main metabolites of Di(2‐ethylhexyl) phthalate (DEHP) in the urine of occupationally or environmentally exposed persons. The present method is, therefore, an addendum to the procedure for the determination of the DEHP metabolites [ Koch et al. 2007].
For calibration, aqueous standard solutions spiked with the target analytes [mono‐n‐butyl phthalate (MnBP) and mono‐iso‐butyl phthalate (MiBP)] and the internal standards (d4‐mono‐n‐butyl phthalate and d4‐mono‐iso‐butyl phthalate) are processed and analyzed in the same way as the urine samples.