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Trichloroethene, 1,1,1-trichloroethane, 1,1,2,2-tetrachloroethane and tetrachloroethene - Determination of trichloroacetic acid in urine using headspace-gas chromatography-mass spectrometry [Biomonitoring Methods, 2017]


Will, W; Bader, M; Berger-Preiss, E; Göen, T; Hartwig, A; MAK Commission; et al; Arand, Michael (2017). Trichloroethene, 1,1,1-trichloroethane, 1,1,2,2-tetrachloroethane and tetrachloroethene - Determination of trichloroacetic acid in urine using headspace-gas chromatography-mass spectrometry [Biomonitoring Methods, 2017]. The MAK Collection for Occupational Health and Safety, 2(1):247-261.

Abstract

Trichloroacetic acid is a metabolite of several chlorinated hydrocarbons which are often used as solvents, such as 1,1,1‐trichloroethane, 1,1,2,2‐tetrachloroethane, tetrachloroethene and above all trichloroethene.
The described method enables the determination of trichloroacetic acid (TCA) in urine and is based on the quantitative, thermolytic degradation of TCA into carbon dioxide and chloroform (trichloromethane) in an aqueous solution. The analyte is chloroform, which can be quantified using headspace‐gas chromatography and a mass selective detector in the Selected‐Ion‐Monitoring‐mode. For quantitation, a calibration is carried out using standard solutions in pooled urine or water which are processed and analysed in the same way as the samples. The validation of the procedure showed a precision in the range of 4.0 to 5.5%. Mean accuracy ranged from 95 to 118%. LOQ was found to be 0.03 mg TCA per liter urine.

Abstract

Trichloroacetic acid is a metabolite of several chlorinated hydrocarbons which are often used as solvents, such as 1,1,1‐trichloroethane, 1,1,2,2‐tetrachloroethane, tetrachloroethene and above all trichloroethene.
The described method enables the determination of trichloroacetic acid (TCA) in urine and is based on the quantitative, thermolytic degradation of TCA into carbon dioxide and chloroform (trichloromethane) in an aqueous solution. The analyte is chloroform, which can be quantified using headspace‐gas chromatography and a mass selective detector in the Selected‐Ion‐Monitoring‐mode. For quantitation, a calibration is carried out using standard solutions in pooled urine or water which are processed and analysed in the same way as the samples. The validation of the procedure showed a precision in the range of 4.0 to 5.5%. Mean accuracy ranged from 95 to 118%. LOQ was found to be 0.03 mg TCA per liter urine.

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Additional indexing

Item Type:Journal Article, not_refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:26 January 2017
Deposited On:17 Oct 2019 11:14
Last Modified:23 Oct 2019 14:55
Publisher:Wiley-VCH Verlag
ISSN:2509-2383
ISBN:9783527600410
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/3527600418.bi7603e2117

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