Header

UZH-Logo

Maintenance Infos

N-Methylformamide and N-methylacetamide in urine [Biomonitoring Methods, 2015]


Will, W; Göen, T; Hartwig, A; MAK Commission; et al; Arand, Michael (2016). N-Methylformamide and N-methylacetamide in urine [Biomonitoring Methods, 2015]. The MAK Collection for Occupational Health and Safety, 1(1):536-553.

Abstract

The present method allows for the determination of the main metabolites of N,N‐dimethylformamide and N,N‐dimethylacetamide in urine: HMMF, NMF, HMMA and NMA, respectively. HMMF and HMMA readily decompose thermally to form N‐methylformamide (NMF) or N‐methylacetamide (NMA) and formaldehyde. Prior to sample injection, thermolysis is carried out for 2 hours at 120°C in order to transform the metabolites completely into the target analytes NMF and NMA. The urine samples are then diluted with ethanol and analyzed using GC–MS. Thus, the sum of HMMF and NMF (DMF metabolites) and the sum of HMMA and NMA (DMA metabolites), respectively, are detected by this procedure.

Abstract

The present method allows for the determination of the main metabolites of N,N‐dimethylformamide and N,N‐dimethylacetamide in urine: HMMF, NMF, HMMA and NMA, respectively. HMMF and HMMA readily decompose thermally to form N‐methylformamide (NMF) or N‐methylacetamide (NMA) and formaldehyde. Prior to sample injection, thermolysis is carried out for 2 hours at 120°C in order to transform the metabolites completely into the target analytes NMF and NMA. The urine samples are then diluted with ethanol and analyzed using GC–MS. Thus, the sum of HMMF and NMF (DMF metabolites) and the sum of HMMA and NMA (DMA metabolites), respectively, are detected by this procedure.

Statistics

Citations

Dimensions.ai Metrics

Altmetrics

Additional indexing

Item Type:Journal Article, not_refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:28 January 2016
Deposited On:17 Oct 2019 10:44
Last Modified:23 Oct 2019 15:15
Publisher:Wiley-VCH Verlag
ISSN:2509-2383
ISBN:9783527600410
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/3527600418.bi6812e2115b

Download

Full text not available from this repository.
View at publisher

Get full-text in a library