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3-Methyl-5-isopropylhydantoin as hemoglobin adduct of N,N-dimethylformamide and methylisocyanate [Biomonitoring Methods, 2015]


Käfferlein, H U; Angerer, J; Leng, G; Gries, W; Hartwig, A; MAK Commission; et al; Arand, Michael (2016). 3-Methyl-5-isopropylhydantoin as hemoglobin adduct of N,N-dimethylformamide and methylisocyanate [Biomonitoring Methods, 2015]. The MAK Collection for Occupational Health and Safety, 1(1):554-577.

Abstract

The analytical method describes the specific determination of 3‐methyl‐5‐isopropylhydantoin (MIH), a hemoglobin adduct of N,N‐dimethylformamide. Following addition of 13C5,15N‐3‐methyl‐5‐isopropylhydantoin (13C5,15N‐MIH) as internal standard, isolated globin is subjected to acid hydrolysis for one hour at 110°C. After pH adjustment (pH 3 – 5) the hydrolysate is extracted twice with ethyl acetate. The combined extracts are washed with sodium hydrogen carbonate solution and dried under a stream of nitrogen. The residue is dissolved in ethyl acetate. An aliquot of the solution is analyzed using GC–MS. For calibration purposes, calibration standards are prepared in pooled globin. They are processed in the same way as the samples to be analyzed.

Abstract

The analytical method describes the specific determination of 3‐methyl‐5‐isopropylhydantoin (MIH), a hemoglobin adduct of N,N‐dimethylformamide. Following addition of 13C5,15N‐3‐methyl‐5‐isopropylhydantoin (13C5,15N‐MIH) as internal standard, isolated globin is subjected to acid hydrolysis for one hour at 110°C. After pH adjustment (pH 3 – 5) the hydrolysate is extracted twice with ethyl acetate. The combined extracts are washed with sodium hydrogen carbonate solution and dried under a stream of nitrogen. The residue is dissolved in ethyl acetate. An aliquot of the solution is analyzed using GC–MS. For calibration purposes, calibration standards are prepared in pooled globin. They are processed in the same way as the samples to be analyzed.

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Additional indexing

Item Type:Journal Article, not_refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:28 January 2016
Deposited On:17 Oct 2019 10:29
Last Modified:23 Oct 2019 15:12
Publisher:Wiley-VCH Verlag
ISSN:2509-2383
ISBN:9783527600410
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/3527600418.bi6812e2115a

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