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S-Alkyl mercapturic acids (methyl mercapturic acid, ethyl mercapturic acid, n-propyl mercapturic acid und iso-propyl mercapturic acid) in urine [Biomonitoring Methods, 2015]


Eckert, E; Göen, T; Hartwig, A; MAK Commission; et al; Arand, Michael (2016). S-Alkyl mercapturic acids (methyl mercapturic acid, ethyl mercapturic acid, n-propyl mercapturic acid und iso-propyl mercapturic acid) in urine [Biomonitoring Methods, 2015]. The MAK Collection for Occupational Health and Safety, 1(1):448-472.

Abstract

The analytical method permits simultaneous determination of various alkyl mercapturic acids in urine. For determination, the urine samples are buffered to pH = 2.5, spiked with isotope labeled internal standards (d3‐MeMA, d5‐EtMA, d7‐PrMA and d7‐iPrMA), processed using online solid phase extraction (online SPE) and analyzed. For that purpose the analytes are enriched on a C18 preconcentration column [Restricted Access Material (RAM) phase] and separated from most of the matrix. Subsequently, the analytes are transferred in backflush mode from the RAM phase to the analytical column, separated chromatographically and finally subjected to tandem mass spectrometric detection. For quantification, a calibration is carried out using standard solutions in pooled urine which are processed and analyzed in the same way as the samples to be analyzed.

Abstract

The analytical method permits simultaneous determination of various alkyl mercapturic acids in urine. For determination, the urine samples are buffered to pH = 2.5, spiked with isotope labeled internal standards (d3‐MeMA, d5‐EtMA, d7‐PrMA and d7‐iPrMA), processed using online solid phase extraction (online SPE) and analyzed. For that purpose the analytes are enriched on a C18 preconcentration column [Restricted Access Material (RAM) phase] and separated from most of the matrix. Subsequently, the analytes are transferred in backflush mode from the RAM phase to the analytical column, separated chromatographically and finally subjected to tandem mass spectrometric detection. For quantification, a calibration is carried out using standard solutions in pooled urine which are processed and analyzed in the same way as the samples to be analyzed.

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Additional indexing

Item Type:Journal Article, not_refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:28 January 2016
Deposited On:17 Oct 2019 10:25
Last Modified:23 Oct 2019 15:11
Publisher:Wiley-VCH Verlag
ISSN:2509-2383
ISBN:9783527600410
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/3527600418.bi10694e2115

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