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Impact of PDGF-BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post-operation


Meier Bürgisser, Gabriella; Evrova, Olivera; Calcagni, Maurizio; Scalera, Chiara; Giovanoli, Pietro; Buschmann, Johanna (2020). Impact of PDGF-BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post-operation. FEBS Open Bio, 10(3):327-337.

Abstract

Current methods for tendon rupture repair suffer from two main drawbacks: insufficient strength and adhesion formation, which lead to re-rupture and impaired gliding. A novel polymer tube may help to overcome these problems by allowing growth factor delivery to the wound site and adhesion reduction, and by acting as a physical barrier to the surrounding tissue. In this study, we used a bi-layered DegraPol® tube to deliver PDGF-BB to the wound site in a rabbit full transection Achilles tendon model. We then performed histological and immunohistochemical analysis at 3 weeks post-operation. Sustained delivery of PDGF-BB to the healing Achilles tendon led to a significantly more homogenous cell distribution within the healing tissue. Lower cell densities next to the implant material were determined for +PDGF-BB samples compared to -PDGF-BB. PDGF-BB application increased proteoglycan content and reduced alpha-SMA areas, clusters of different sizes, mainly vessels. Finally, PDGF-BB reduced collagen I and III in the extracellular matrix. The sustained delivery of PDGF-BB via an electrospun DegraPol® tube accelerated tendon wound healing by causing a more uniform cell distribution with higher proteoglycan content and less fibrotic tissue. Moreover, the application of this growth factor reduced collagen III and alpha-SMA, indicating faster and less fibrotic tendon healing.

Abstract

Current methods for tendon rupture repair suffer from two main drawbacks: insufficient strength and adhesion formation, which lead to re-rupture and impaired gliding. A novel polymer tube may help to overcome these problems by allowing growth factor delivery to the wound site and adhesion reduction, and by acting as a physical barrier to the surrounding tissue. In this study, we used a bi-layered DegraPol® tube to deliver PDGF-BB to the wound site in a rabbit full transection Achilles tendon model. We then performed histological and immunohistochemical analysis at 3 weeks post-operation. Sustained delivery of PDGF-BB to the healing Achilles tendon led to a significantly more homogenous cell distribution within the healing tissue. Lower cell densities next to the implant material were determined for +PDGF-BB samples compared to -PDGF-BB. PDGF-BB application increased proteoglycan content and reduced alpha-SMA areas, clusters of different sizes, mainly vessels. Finally, PDGF-BB reduced collagen I and III in the extracellular matrix. The sustained delivery of PDGF-BB via an electrospun DegraPol® tube accelerated tendon wound healing by causing a more uniform cell distribution with higher proteoglycan content and less fibrotic tissue. Moreover, the application of this growth factor reduced collagen III and alpha-SMA, indicating faster and less fibrotic tendon healing.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:1 March 2020
Deposited On:01 Nov 2019 13:40
Last Modified:03 Mar 2020 02:03
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:2211-5463
OA Status:Green
Publisher DOI:https://doi.org/10.1002/2211-5463.12736
PubMed ID:31571428

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