The aim of the study was to develop a new flow cytometric assay for the determination of the bacterial count in commercially processed boar semen. In total 224 fresh boar semen samples collected at an AI-station were analyzed. The number of total viable counts (TVC) was determined by using flow cytometry after staining with SYBR Green I and Propidium Iodide (PI). In the first part of the study 111 fresh boar semen samples were spiked with pure cultures of defined numbers of bacteria commonly detected in boar ejaculates and analyzed by flow cytometry. In the second part, 113 fresh semen samples were assessed on the day of collection through flow cytometry and the Most Probable Number (MPN) method, as the standard bacteriological method. The first part of the study showed a strong correlation between the detected and expected numbers (r = 0.96; P < 0.001), while in the second part of the study the TVC determined by flow cytometry and by the MPN method correlated only moderately (r = 0.28; P < 0.01; median MPN: 24,000 ± MAD 21,600 bacteria/mL; median flow cytometry: 24,426 ± MAD 15,610 bacteria/mL). In summary flow cytometry is a fast alternative to the classical culture technique to determine highly contaminated boar ejaculates. The developed flow cytometric protocol enables one to enumerate the viable bacteria within fresh boar ejaculates without requiring numerous treatment steps, and thus offering the possibility of an on-line use in AI-centers.