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Evaluation of the AID AmpC line probe assay for molecular detection of AmpC-producing Enterobacterales


Wagner, Karoline; Mancini, Stefano; Ritter, Claudia; Böttger, Erik C; Keller, Peter M (2019). Evaluation of the AID AmpC line probe assay for molecular detection of AmpC-producing Enterobacterales. Journal of Global Antimicrobial Resistance, 19:8-13.

Abstract

OBJECTIVES

In this study, the commercially available AID AmpC line probe assay (LPA) was evaluated for detection of plasmid-mediatedbla$_{AmpC}$ β-lactamase genes in Enterobacterales as well as chromosomal mutations in the bla$_{AmpC}$ promoter/attenuator regions in Escherichia coli.

METHODS

Accuracy of the AID AmpC probes was assessed using Enterobacterales clinical isolates harbouring diverse plasmid-mediated AmpC enzymes (ACC, ACT, DHA, FOX, CMY and MOX) and E. coli clinical isolates with mutations in the chromosomal bla$_{AmpC}$ promoter/attenuator regions. The diagnostic performance of the AID AmpC LPA for bla$_{AmpC}$ detection directly from clinical specimens was determined using 99 clinical urine specimens with bacterial cell counts >10$^{5}$CFU/mL and the results were compared with culture-based phenotypic drug susceptibility testing (DST).

RESULTS

Detection of bla$_{AmpC}$ genes in Enterobacterales clinical isolates showed 100% congruence with phenotypic DST results. The AID AmpC LPA showed 100% specificity [95% confidence interval (CI) 96-100%] and 100% sensitivity (95% CI 75-100%) for detection of plasmid-meditated bla$_{AmpC}$ and E. coli genomic bla$_{AmpC}$ promoter/attenuator mutations directly from clinical urine specimens. The AID AmpC LPA detected three AmpC-producers in urine specimens with bacterial cell counts >10$^{5}$CFU/mL that were missed by culture-based phenotypic DST, thereby displaying higher diagnostic sensitivity.

CONCLUSION

The AID AmpC LPA is an accurate, sensitive and easy-to-use test that can be readily implemented in any diagnostic laboratory for molecular detection of bla$_{AmpC}$ genes in Enterobacterales.

Abstract

OBJECTIVES

In this study, the commercially available AID AmpC line probe assay (LPA) was evaluated for detection of plasmid-mediatedbla$_{AmpC}$ β-lactamase genes in Enterobacterales as well as chromosomal mutations in the bla$_{AmpC}$ promoter/attenuator regions in Escherichia coli.

METHODS

Accuracy of the AID AmpC probes was assessed using Enterobacterales clinical isolates harbouring diverse plasmid-mediated AmpC enzymes (ACC, ACT, DHA, FOX, CMY and MOX) and E. coli clinical isolates with mutations in the chromosomal bla$_{AmpC}$ promoter/attenuator regions. The diagnostic performance of the AID AmpC LPA for bla$_{AmpC}$ detection directly from clinical specimens was determined using 99 clinical urine specimens with bacterial cell counts >10$^{5}$CFU/mL and the results were compared with culture-based phenotypic drug susceptibility testing (DST).

RESULTS

Detection of bla$_{AmpC}$ genes in Enterobacterales clinical isolates showed 100% congruence with phenotypic DST results. The AID AmpC LPA showed 100% specificity [95% confidence interval (CI) 96-100%] and 100% sensitivity (95% CI 75-100%) for detection of plasmid-meditated bla$_{AmpC}$ and E. coli genomic bla$_{AmpC}$ promoter/attenuator mutations directly from clinical urine specimens. The AID AmpC LPA detected three AmpC-producers in urine specimens with bacterial cell counts >10$^{5}$CFU/mL that were missed by culture-based phenotypic DST, thereby displaying higher diagnostic sensitivity.

CONCLUSION

The AID AmpC LPA is an accurate, sensitive and easy-to-use test that can be readily implemented in any diagnostic laboratory for molecular detection of bla$_{AmpC}$ genes in Enterobacterales.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:30 April 2019
Deposited On:07 Jan 2020 11:04
Last Modified:07 Jan 2020 12:08
Publisher:Elsevier
ISSN:2213-7165
OA Status:Green
Publisher DOI:https://doi.org/10.1016/j.jgar.2019.04.015
PubMed ID:31051288

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