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Scattering lens for structured illumination microscopy


Malavalli, Archana; Aegerter, Christof M (2019). Scattering lens for structured illumination microscopy. OSA Continuum, 2(11):2997.

Abstract

In this article, we describe a way to obtain beyond diffraction limited imaging capability behind a scattering medium using structured illumination microscopy (SIM). A scattering medium such as a ground glass diffuser or a biological material poses obstacles to image formation, let alone high resolution imaging. However, building on the work of Vellekoop et al. [Opt. Lett. 35, 1245 (2010). [CrossRef] ] that transforms a scattering medium into a lens, we have previously demonstrated a technique to generate an array of focal spots behind a scattering medium [Opt. Express 24, 23018 (2016). [CrossRef] ]. Using such focal spot arrays, we illuminate fluorescent beads hidden behind a scattering medium. We process the recorded fluorescence images using an SIM reconstruction algorithm to reveal images beyond the limit of resolution of the scattering lens.

Abstract

In this article, we describe a way to obtain beyond diffraction limited imaging capability behind a scattering medium using structured illumination microscopy (SIM). A scattering medium such as a ground glass diffuser or a biological material poses obstacles to image formation, let alone high resolution imaging. However, building on the work of Vellekoop et al. [Opt. Lett. 35, 1245 (2010). [CrossRef] ] that transforms a scattering medium into a lens, we have previously demonstrated a technique to generate an array of focal spots behind a scattering medium [Opt. Express 24, 23018 (2016). [CrossRef] ]. Using such focal spot arrays, we illuminate fluorescent beads hidden behind a scattering medium. We process the recorded fluorescence images using an SIM reconstruction algorithm to reveal images beyond the limit of resolution of the scattering lens.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Physics Institute
Dewey Decimal Classification:530 Physics
Language:English
Date:15 November 2019
Deposited On:09 Jan 2020 11:01
Last Modified:11 Apr 2022 15:08
Publisher:Optical Society of America
ISSN:2578-7519
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1364/osac.2.002997
Project Information:
  • : FunderSNSF
  • : Grant IDCR23I2_166556
  • : Project TitleThe Biomechanics of developing tissues - soft matter in action

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