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Serum lipidome analysis of healthy beagle dogs receiving different diets


Boretti, Felicitas S; Burla, Bo; Deuel, Jeremy; Gao, Liang; Wenk, Markus R; Liesegang, Annette; Sieber-Ruckstuhl, Nadja S (2020). Serum lipidome analysis of healthy beagle dogs receiving different diets. Metabolomics, 16(1):1.

Abstract

INTRODUCTION: Food and dietary ingredients have significant effects on metabolism and health.
OBJECTIVE: To evaluate whether and how different diets affected the serum lipidomic profile of dogs.
METHODS: Sixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography-mass spectrometry.
RESULTS: Dogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.
CONCLUSION: This study found that different diets had significant effects on the dog's serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.

Abstract

INTRODUCTION: Food and dietary ingredients have significant effects on metabolism and health.
OBJECTIVE: To evaluate whether and how different diets affected the serum lipidomic profile of dogs.
METHODS: Sixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography-mass spectrometry.
RESULTS: Dogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.
CONCLUSION: This study found that different diets had significant effects on the dog's serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Animal Nutrition
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Scopus Subject Areas:Health Sciences > Endocrinology, Diabetes and Metabolism
Life Sciences > Biochemistry
Life Sciences > Clinical Biochemistry
Uncontrolled Keywords:Canine; Fatty acids; Lipid metabolism; Mass spectrometry; PUFA
Language:English
Date:1 January 2020
Deposited On:07 Jan 2020 15:48
Last Modified:29 Jul 2020 12:24
Publisher:Springer
ISSN:1573-3882
OA Status:Hybrid
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1007/s11306-019-1621-3
PubMed ID:31797205

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