Header

UZH-Logo

Maintenance Infos

Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions


Jimenez-Rojo, Lucia; Mitsiadis, Thimios A (2019). Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions. In: Papagerakis, Petros. Odontogenesis. Methods and Protocols. New York: Springer, 49-55.

Abstract

Tissue interactions are crucial during the development of organs. Among the most studied tissue interactions are those that take place between the epithelial cells and the underlying mesenchymal cells, known as epithelial-mesenchymal interactions. Tissue recombination assay is a valuable model to study the mechanisms involved in the regulation of such interactions. Here, we describe how to dissociate and recombine the epithelial and mesenchymal components of the embryonic tooth. In addition, we explain how to transplant the recombined tissues under the kidney capsule of immunocompromised mice in order to allow their further development into a mature tooth.

Abstract

Tissue interactions are crucial during the development of organs. Among the most studied tissue interactions are those that take place between the epithelial cells and the underlying mesenchymal cells, known as epithelial-mesenchymal interactions. Tissue recombination assay is a valuable model to study the mechanisms involved in the regulation of such interactions. Here, we describe how to dissociate and recombine the epithelial and mesenchymal components of the embryonic tooth. In addition, we explain how to transplant the recombined tissues under the kidney capsule of immunocompromised mice in order to allow their further development into a mature tooth.

Statistics

Citations

Dimensions.ai Metrics

Altmetrics

Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Language:English
Date:2019
Deposited On:14 Feb 2020 09:09
Last Modified:15 Apr 2021 15:11
Publisher:Springer
Series Name:Methods in Molecular Biology
ISSN:1064-3745
ISBN:978-1-4939-9011-5
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-9012-2_6
Related URLs:https://doi.org/10.1007/978-1-4939-9012-2 (Publisher)
PubMed ID:30838564

Download

Full text not available from this repository.
View at publisher

Get full-text in a library