Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Pleiotropic combinatorial transcriptomes of human breast cancer cells exposed to mixtures of dietary phytoestrogens

Dip, R; Lenz, S; Gmuender, H; Naegeli, H (2009). Pleiotropic combinatorial transcriptomes of human breast cancer cells exposed to mixtures of dietary phytoestrogens. Food and Chemical Toxicology, 47(4):787-795.

Abstract

Combinations of estrogen receptor agonists have been shown to exert more potent effects than single compounds in many single-endpoint bioassays. However, to our knowledge, it has never been tested how genome-wide expression programs are shaped by the interplay of multiple estrogenic stimuli. In view of the abundance of dietary phytoestrogens, we selected binary mixtures of these phytochemicals to determine their global impact using high-density DNA microarrays. MCF7 cells, a frequent in vitro model for molecular processes associated with breast cancer, were exposed to a sub-saturating concentration of coumestrol either alone or in combination with analogs that exhibit 1000-fold lower estrogen receptor activity. As expected, in the presence of coumestrol, the induction of many estrogen-sensitive genes was not further increased by the addition of resveratrol or enterolactone. However, it was surprising to find that these weak phytoestrogens, when combined with coumestrol in equal concentrations, were able to more than double the number of significantly regulated transcripts. Thus, phytoestrogens with low receptor affinity interact with other estrogenic agonists to generate more widespread expression fingerprints. This effect involving the number of susceptible transcripts instead of their amplitude of induction remains undetected if mixtures are evaluated with conventional bioassays.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Veterinary Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Food Science
Life Sciences > Toxicology
Language:English
Date:2009
Deposited On:31 Mar 2009 13:55
Last Modified:02 Nov 2024 02:40
Publisher:Elsevier
ISSN:0278-6915
Funders:Swiss National Science Foundation
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.fct.2009.01.008
PubMed ID:19167446
Project Information:
  • Funder: SNSF
  • Grant ID:
  • Project Title: Swiss National Science Foundation

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
15 citations in Web of Science®
16 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

2 downloads since deposited on 31 Mar 2009
0 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications