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Intraluminal vesicles of binucleate trophoblast cell granules are a possible source of placental exosomes in ruminants


Klisch, Karl; Schraner, Elisabeth M (2020). Intraluminal vesicles of binucleate trophoblast cell granules are a possible source of placental exosomes in ruminants. Placenta, 90:58-61.

Abstract

Introduction: Exosomes are membrane-bound small extracellular vesicles, which play important roles in intercellular communication, including the feto-maternal communication. Placenta-derived exosomes have been identified in maternal blood of a variety of species, including cattle and sheep.
Methods: Transmission electron microscopy is used to characterize intraluminal vesicles in binucleate trophoblast cell secretory granules and extracellular vesicles in placentome samples from eight ruminant species of the bovidae and cervidae clades.
Results: In all species the secretory granules of binucleate cells contain intraluminal vesicles of 40–70 nm diameter. After fusion of the binucleate trophoblast cells with cells of the uterine epithelium these vesicles are exocytosed together with the granule's secretory proteins. The vesicles are located at the basement membrane of the uterine epithelium and in the connective tissue underneath.
Discussion: We suggest that these vesicles function as exosomes. Their function might be either locally in the maternal endometrial stroma or they could have systemic functions after entering the maternal blood. Earlier electron microscopical studies in other ruminants, including species of the most basic ruminant clade (tragulidae), indicate that the intraluminal vesicles are a general feature of ruminant binucleate trophoblast cell granules. Our findings suggest that ruminant BNC are a source of exosomes, which are released into the maternal organism and are thus a newly described type of feto-maternal communication in ruminants.

Abstract

Introduction: Exosomes are membrane-bound small extracellular vesicles, which play important roles in intercellular communication, including the feto-maternal communication. Placenta-derived exosomes have been identified in maternal blood of a variety of species, including cattle and sheep.
Methods: Transmission electron microscopy is used to characterize intraluminal vesicles in binucleate trophoblast cell secretory granules and extracellular vesicles in placentome samples from eight ruminant species of the bovidae and cervidae clades.
Results: In all species the secretory granules of binucleate cells contain intraluminal vesicles of 40–70 nm diameter. After fusion of the binucleate trophoblast cells with cells of the uterine epithelium these vesicles are exocytosed together with the granule's secretory proteins. The vesicles are located at the basement membrane of the uterine epithelium and in the connective tissue underneath.
Discussion: We suggest that these vesicles function as exosomes. Their function might be either locally in the maternal endometrial stroma or they could have systemic functions after entering the maternal blood. Earlier electron microscopical studies in other ruminants, including species of the most basic ruminant clade (tragulidae), indicate that the intraluminal vesicles are a general feature of ruminant binucleate trophoblast cell granules. Our findings suggest that ruminant BNC are a source of exosomes, which are released into the maternal organism and are thus a newly described type of feto-maternal communication in ruminants.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Health Sciences > Reproductive Medicine
Health Sciences > Obstetrics and Gynecology
Life Sciences > Developmental Biology
Uncontrolled Keywords:Obstetrics and Gynaecology, Developmental Biology, Reproductive Medicine
Language:English
Date:1 January 2020
Deposited On:20 Jan 2020 17:00
Last Modified:29 Jul 2020 13:00
Publisher:Elsevier
ISSN:0143-4004
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.placenta.2019.12.006

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