Header

UZH-Logo

Maintenance Infos

Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia


Sathe, Santosh; Mathew, Anugraha; Agnoli, Kirsty; Eberl, Leo; Kümmerli, Rolf (2019). Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia. Evolution Letters, 3(6):610-622.

Abstract

Explaining how cooperation can persist in the presence of cheaters, exploiting the cooperative acts, is a challenge for evolutionary biology. Microbial systems have proved extremely useful to test evolutionary theory and identify mechanisms maintaining cooperation. One of the most widely studied system is the secretion and sharing of iron-scavenging siderophores by Pseudomonas bacteria, with many insights gained from this system now being considered as hallmarks of bacterial cooperation. Here, we introduce siderophore secretion by the bacterium Burkholderia cenocepacia H111 as a novel parallel study system, and show that this system behaves differently. For ornibactin, the main siderophore of this species, we discovered a novel mechanism of how cheating can be prevented. Particularly, we found that secreted ornibactin cannot be exploited by ornibactin-defective mutants because ornibactin receptor and synthesis genes are co-expressed from the same operon, such that disruptive mutations in synthesis genes compromise receptor availability required for siderophore uptake and cheating. For pyochelin, the secondary siderophore of this species, we found that cheating was possible, but the relative success of cheaters was positive frequency-dependent, thus diametrically opposite to the Pseudomonas and other microbial systems. Altogether, our results highlight that expanding our repertoire of microbial study systems leads to new discoveries and suggest that there is an enormous diversity of social interactions out there in nature, and we might have only looked at the tip of the iceberg so far.

Abstract

Explaining how cooperation can persist in the presence of cheaters, exploiting the cooperative acts, is a challenge for evolutionary biology. Microbial systems have proved extremely useful to test evolutionary theory and identify mechanisms maintaining cooperation. One of the most widely studied system is the secretion and sharing of iron-scavenging siderophores by Pseudomonas bacteria, with many insights gained from this system now being considered as hallmarks of bacterial cooperation. Here, we introduce siderophore secretion by the bacterium Burkholderia cenocepacia H111 as a novel parallel study system, and show that this system behaves differently. For ornibactin, the main siderophore of this species, we discovered a novel mechanism of how cheating can be prevented. Particularly, we found that secreted ornibactin cannot be exploited by ornibactin-defective mutants because ornibactin receptor and synthesis genes are co-expressed from the same operon, such that disruptive mutations in synthesis genes compromise receptor availability required for siderophore uptake and cheating. For pyochelin, the secondary siderophore of this species, we found that cheating was possible, but the relative success of cheaters was positive frequency-dependent, thus diametrically opposite to the Pseudomonas and other microbial systems. Altogether, our results highlight that expanding our repertoire of microbial study systems leads to new discoveries and suggest that there is an enormous diversity of social interactions out there in nature, and we might have only looked at the tip of the iceberg so far.

Statistics

Citations

Dimensions.ai Metrics

Altmetrics

Downloads

5 downloads since deposited on 20 Jan 2020
5 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Department of Quantitative Biomedicine
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:1 December 2019
Deposited On:20 Jan 2020 10:27
Last Modified:24 Feb 2020 14:13
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:2056-3744
OA Status:Gold
Free access at:Official URL. An embargo period may apply.
Publisher DOI:https://doi.org/10.1002/evl3.144
PubMed ID:PMC6906993
Project Information:
  • : FunderSNSF
  • : Grant ID31003A_169307
  • : Project TitleCommunication in bacterial biofilms
  • : FunderSNSF
  • : Grant IDPP00P3_165835
  • : Project TitleCooperation and competition in bacteria: extension
  • : FunderH2020
  • : Grant ID681295
  • : Project TitleBacterial cooperation at the individual cell level

Download

Gold Open Access

Download PDF  'Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia'.
Preview
Content: Published Version
Language: English
Filetype: PDF
Size: 1MB
View at publisher
Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)