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Influence of age, group size and the presence of porcine reproductive and respiratory syndrome virus on the collection of oral fluids


Graage, Robert; Hennig-Pauka, Isabel; Arbinger, Heidelore; Ritzmann, Mathias; Ladinig, Andrea (2019). Influence of age, group size and the presence of porcine reproductive and respiratory syndrome virus on the collection of oral fluids. Veterinary Journal, 244:13-15.

Abstract

Pen-based oral fluid (OF) samples have been approved for detecting porcine reproductive and respiratory syndrome virus (PRRSV) RNA and antibodies. The aim of the study was to investigate the influence of age, group size and the presence of PRRSV infection on sampling using pen-based OF collected by presenting pigs with a rope to chew. Samples were collected from pigs of 4/5 weeks of age, 7/8 weeks of age and 12/14 weeks of age. Subsequent PRRSV-PCR and ELISA analyses of OF and serum samples were performed. In this study, 76.5-81.9% of pigs chewed the rope. The group size had significant impact on the pig-rope-interaction (P=0.047). OF and serum had an almost perfect and significant agreement for the detection of antibodies (κ=0.829, P<0.001) and a fair and not significant agreement (κ=0.347, P=0.75) for the detection PRRSV-genome.

Abstract

Pen-based oral fluid (OF) samples have been approved for detecting porcine reproductive and respiratory syndrome virus (PRRSV) RNA and antibodies. The aim of the study was to investigate the influence of age, group size and the presence of PRRSV infection on sampling using pen-based OF collected by presenting pigs with a rope to chew. Samples were collected from pigs of 4/5 weeks of age, 7/8 weeks of age and 12/14 weeks of age. Subsequent PRRSV-PCR and ELISA analyses of OF and serum samples were performed. In this study, 76.5-81.9% of pigs chewed the rope. The group size had significant impact on the pig-rope-interaction (P=0.047). OF and serum had an almost perfect and significant agreement for the detection of antibodies (κ=0.829, P<0.001) and a fair and not significant agreement (κ=0.347, P=0.75) for the detection PRRSV-genome.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Uncontrolled Keywords:Animal Science and Zoology, General Veterinary, ELISA; Oral fluid; PCR; PRRSV; Rope; Surveillance
Language:English
Date:1 February 2019
Deposited On:21 Jan 2020 16:46
Last Modified:22 Jan 2020 08:38
Publisher:Elsevier
ISSN:1090-0233
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.tvjl.2018.12.005
PubMed ID:30825888

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