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3D Manipulation and Imaging of Plant Cells using Acoustically Activated Microbubbles


Läubli, Nino F; Shamsudhin, Naveen; Vogler, Hannes; Munglani, Gautam; Grossniklaus, Ueli; Ahmed, Daniel; Nelson, Bradley J (2019). 3D Manipulation and Imaging of Plant Cells using Acoustically Activated Microbubbles. Small Methods, 3(3):1800527.

Abstract

The precise manipulation of single cells and organisms opens exciting new possibilities for biological research. In this work, an acoustic rotational manipulation method for imaging single cells of different plant species (pollen grains of Lilium longiflorum and Arabidopsis thaliana) is demonstrated. Acoustically activated microbubbles generate radiation forces as well as microvortices in the aqueous medium, which allow various specimens to be trapped and precisely rotated. The rotational behavior of individual plant cells is studied and their motion to facilitate 3D fluorescent microscopy is controlled. The use of this manipulation technique for high‐resolution 3D optical reconstructions of nontransparent samples is demonstrated. The applicability of this method for open‐microchannel arrangement, which may enable multiplexed 3D access to samples for microsurgery and injection, is further demonstrated.

Abstract

The precise manipulation of single cells and organisms opens exciting new possibilities for biological research. In this work, an acoustic rotational manipulation method for imaging single cells of different plant species (pollen grains of Lilium longiflorum and Arabidopsis thaliana) is demonstrated. Acoustically activated microbubbles generate radiation forces as well as microvortices in the aqueous medium, which allow various specimens to be trapped and precisely rotated. The rotational behavior of individual plant cells is studied and their motion to facilitate 3D fluorescent microscopy is controlled. The use of this manipulation technique for high‐resolution 3D optical reconstructions of nontransparent samples is demonstrated. The applicability of this method for open‐microchannel arrangement, which may enable multiplexed 3D access to samples for microsurgery and injection, is further demonstrated.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:1 March 2019
Deposited On:04 Feb 2020 12:42
Last Modified:29 Jul 2020 13:18
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:2366-9608
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/smtd.201800527
Project Information:
  • : FunderSNSF
  • : Grant IDCR22I2_166110
  • : Project TitleMechanical Basis for the Convergent Evolution of Sensory Hairs in Animals and Plants

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