Header

UZH-Logo

Maintenance Infos

Noradrenaline Increases the Expression of the Proenkephalin Gene in Cultured Astroglial Cells by Acting on β1- and α1-Adrenoceptors


Hildebrand, B; Olenik, C; Theodoridu, A; Boeckh, C; Uhl, A; Meyer, D K (2002). Noradrenaline Increases the Expression of the Proenkephalin Gene in Cultured Astroglial Cells by Acting on β1- and α1-Adrenoceptors. Journal of Neurochemistry, 66(5):1860-1867.

Abstract

In primary culture, type I astroglial cells from neocortex of newborn rats express the proenkephalin gene. The glial cells are not homogeneous but differ in their morphology; i.e., polygonal and process‐bearing cells are found. Transcription of the proenkephalin gene is increased via protein kinase A upon stimulation with cyclic AMP (cAMP) analogues. In the present study, how noradrenaline affected the expression of the proenkephalin gene in both cell types was investigated. Noradrenaline enhanced the levels of proenkephalin mRNA in a concentration‐dependent manner. Experiments with subtype‐selective antagonists suggested that β1‐adrenoceptors were involved. In situ hybridization showed that proenkephalin mRNA was induced only in polygonal cells. Noradrenaline also increased the levels of cAMP. However, concentrations of noradrenaline that produced a maximal increase in cAMP caused only submaximal elevations of proenkephalin mRNA. This discrepancy was explained by the finding that noradrenaline increased the expression of the proenkephalin gene also via α1‐adrenoceptors. It is concluded that β1‐ and α1‐adrenoceptors can act in a synergistic manner on the expression of the proenkephalin gene in astroglial cells.

Abstract

In primary culture, type I astroglial cells from neocortex of newborn rats express the proenkephalin gene. The glial cells are not homogeneous but differ in their morphology; i.e., polygonal and process‐bearing cells are found. Transcription of the proenkephalin gene is increased via protein kinase A upon stimulation with cyclic AMP (cAMP) analogues. In the present study, how noradrenaline affected the expression of the proenkephalin gene in both cell types was investigated. Noradrenaline enhanced the levels of proenkephalin mRNA in a concentration‐dependent manner. Experiments with subtype‐selective antagonists suggested that β1‐adrenoceptors were involved. In situ hybridization showed that proenkephalin mRNA was induced only in polygonal cells. Noradrenaline also increased the levels of cAMP. However, concentrations of noradrenaline that produced a maximal increase in cAMP caused only submaximal elevations of proenkephalin mRNA. This discrepancy was explained by the finding that noradrenaline increased the expression of the proenkephalin gene also via α1‐adrenoceptors. It is concluded that β1‐ and α1‐adrenoceptors can act in a synergistic manner on the expression of the proenkephalin gene in astroglial cells.

Statistics

Citations

Dimensions.ai Metrics
4 citations in Web of Science®
5 citations in Scopus®
Google Scholar™

Altmetrics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Psychiatric University Hospital Zurich > Clinic for Psychiatry, Psychotherapy, and Psychosomatics
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:23 November 2002
Deposited On:19 Feb 2020 10:43
Last Modified:31 Jul 2020 03:45
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:0022-3042
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1046/j.1471-4159.1996.66051860.x
PubMed ID:8780011

Download

Full text not available from this repository.
View at publisher

Get full-text in a library