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Compounds from Olea europaea and Pistacia lentiscus inhibit oral microbial growth


Karygianni, Lamprini; Cecere, Manuel; Argyropoulou, Aikaterini; Hellwig, Elmar; Skaltsounis, Alexios Leandros; Wittmer, Annette; Tchorz, Jörg Philipp; Al-Ahmad, Ali (2019). Compounds from Olea europaea and Pistacia lentiscus inhibit oral microbial growth. BMC Complementary and Alternative Medicine, 19:51.

Abstract

BACKGROUND
In view of the increasing antibiotic resistance, the introduction of natural anti-infective agents has brought a new era in the treatment of bacterially derived oral diseases.
METHODS
The aim of this study was to investigate the antimicrobial potential of five natural constituents of Olea europaea (oleuropein, maslinic acid, hydroxytyrosol, oleocanthal, oleacein) and three compounds of Pistacia lentiscus (24Z-isomasticadienolic acid, oleanolic acid, oleanonic aldehyde) against ten representative oral bacterial species and a Candida albicans strain. After the isolation and quality control of natural compounds, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay were performed.
RESULTS
Among all O. europaea-derived constituents, maslinic acid was the most active (MIC = 4.9-312 μg mL$^{- 1}$, MBC = 9.8-25 μg mL$^{- 1}$) one against oral streptococci and anaerobic pathogenic bacteria (Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra), while oleuropein, hydroxytyrosol, oleocanthal and oleacein showed milder, yet significant effects against P. gingivalis and F. nucleatum. Among all P. lentiscus compounds, oleanolic acid was the most effective one against almost all microorganisms with MIC values ranging from 9.8 μg mL$^{- 1}$ (P. gingivalis) to 625 μg mL$^{- 1}$ (F. nucleatum, P. micra). In the presence of 24Z-isomasticadienolic acid, a mean inhibitory concentration range of 2.4 μg mL$^{- 1}$ to 625 μg mL$^{- 1}$ was observed for strict anaerobia. The MIC value for 24Z-isomasticadienolic acid was estimated between 39 μg mL$^{- 1}$ (Streptococcus sobrinus, Streptococcus oralis) and 78 μg mL$^{- 1}$ (Streptococcus mutans). All tested compounds showed no effects against Prevotella intermedia.
CONCLUSIONS
Overall, maslinic acid and oleanolic acid exerted the most significant inhibitory activity against the tested oral pathogens, especially streptococci and anaerobic oral microorganisms.

Abstract

BACKGROUND
In view of the increasing antibiotic resistance, the introduction of natural anti-infective agents has brought a new era in the treatment of bacterially derived oral diseases.
METHODS
The aim of this study was to investigate the antimicrobial potential of five natural constituents of Olea europaea (oleuropein, maslinic acid, hydroxytyrosol, oleocanthal, oleacein) and three compounds of Pistacia lentiscus (24Z-isomasticadienolic acid, oleanolic acid, oleanonic aldehyde) against ten representative oral bacterial species and a Candida albicans strain. After the isolation and quality control of natural compounds, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay were performed.
RESULTS
Among all O. europaea-derived constituents, maslinic acid was the most active (MIC = 4.9-312 μg mL$^{- 1}$, MBC = 9.8-25 μg mL$^{- 1}$) one against oral streptococci and anaerobic pathogenic bacteria (Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra), while oleuropein, hydroxytyrosol, oleocanthal and oleacein showed milder, yet significant effects against P. gingivalis and F. nucleatum. Among all P. lentiscus compounds, oleanolic acid was the most effective one against almost all microorganisms with MIC values ranging from 9.8 μg mL$^{- 1}$ (P. gingivalis) to 625 μg mL$^{- 1}$ (F. nucleatum, P. micra). In the presence of 24Z-isomasticadienolic acid, a mean inhibitory concentration range of 2.4 μg mL$^{- 1}$ to 625 μg mL$^{- 1}$ was observed for strict anaerobia. The MIC value for 24Z-isomasticadienolic acid was estimated between 39 μg mL$^{- 1}$ (Streptococcus sobrinus, Streptococcus oralis) and 78 μg mL$^{- 1}$ (Streptococcus mutans). All tested compounds showed no effects against Prevotella intermedia.
CONCLUSIONS
Overall, maslinic acid and oleanolic acid exerted the most significant inhibitory activity against the tested oral pathogens, especially streptococci and anaerobic oral microorganisms.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:26 February 2019
Deposited On:31 Jan 2020 10:00
Last Modified:01 Feb 2020 18:09
Publisher:BioMed Central
ISSN:1472-6882
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1186/s12906-019-2461-4
PubMed ID:30808354

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