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Bone marrow chimeras-a vital tool in basic and translational research


Ferreira, Filipa M; Palle, Pushpalatha; vom Berg, Johannes; Prajwal, Prajwal; Laman, Jon D; Buch, Thorsten (2019). Bone marrow chimeras-a vital tool in basic and translational research. Journal of Molecular Medicine, 97(7):889-896.

Abstract

Bone marrow chimeras are used routinely in immunology research as well as in other fields of biology. Here, we provide a concise state-of-the-art review about the types of chimerisms that can be achieved and the type of information that each model generates. We include separate sections for caveats and future developments. We provide examples from the literature in which different types of chimerism were employed to answer specific questions. While simple bone marrow chimeras allow to dissect the role of genes in distinct cell populations such as the hematopoietic cells versus non-hematopoietic cells, mixed bone marrow chimeras can provide detailed information about hematopoietic cell types and the intrinsic and extrinsic roles of individual genes. The advantages and caveats of bone marrow chimerism for the study of microglia are addressed, as well as alternatives to irradiation that minimize blood-brain-barrier disruption. Elementary principles are introduced and their potential is exemplified through summarizing recent studies.

Abstract

Bone marrow chimeras are used routinely in immunology research as well as in other fields of biology. Here, we provide a concise state-of-the-art review about the types of chimerisms that can be achieved and the type of information that each model generates. We include separate sections for caveats and future developments. We provide examples from the literature in which different types of chimerism were employed to answer specific questions. While simple bone marrow chimeras allow to dissect the role of genes in distinct cell populations such as the hematopoietic cells versus non-hematopoietic cells, mixed bone marrow chimeras can provide detailed information about hematopoietic cell types and the intrinsic and extrinsic roles of individual genes. The advantages and caveats of bone marrow chimerism for the study of microglia are addressed, as well as alternatives to irradiation that minimize blood-brain-barrier disruption. Elementary principles are introduced and their potential is exemplified through summarizing recent studies.

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Additional indexing

Contributors:Carla Rohrer-Bley supported us with her expertise in radiation biology
Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Laboratory Animal Science
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Genetics(clinical), Molecular Medicine, Drug Discovery, Bone marrow chimeras; Hematopoiesis; Irradiation damage; Stem cells; Tools
Language:English
Date:1 July 2019
Deposited On:28 Jan 2020 16:16
Last Modified:28 Jan 2020 16:18
Publisher:Springer
ISSN:0946-2716
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/s00109-019-01783-z
PubMed ID:31028417
Project Information:
  • : FunderDutch MS Research Society and the Zabawas foundation
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  • : FunderSwiss MS Society
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  • : FunderHertie Foundation
  • : Grant IDP1150013
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  • : FunderSwiss Cancer Research
  • : Grant IDKFS-4146-02-2017
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  • : Fundere Novartis foundation for medicalbiological research
  • : Grant ID16C231
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  • : FunderUZH EntrepreneurFellowship in BioTech and MedTech
  • : Grant IDMEDEF18-012
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