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Delivery of antibodies into the murine brain via convection-enhanced delivery


Beffinger, Michal Mateusz; Schellhammer, Linda; Pantelyushin, Stanislav; vom Berg, Johannes (2019). Delivery of antibodies into the murine brain via convection-enhanced delivery. Journal of Visualized Experiments (Jove), (149):e59675.

Abstract

Convection-enhanced delivery (CED) is a neurosurgical technique enabling effective perfusion of large brain volumes using a catheter system. Such an approach provides a safe delivery method by-passing the blood brain barrier (BBB), thus allowing treatment with therapeutics with poor BBB-permeability or those for which systemic exposure is not desired, e.g., due to toxicity. CED requires optimization of the catheter design, injection protocol, and properties of the infusate. With this protocol we describe how to perform CED of a solution containing up to 20 µg of an antibody into the caudate putamen of mice. It describes preparation of step catheters, testing them in vitro and performing the CED in mice using a ramping injection program. The protocol can be readily adjusted for other infusion volumes and can be used for injecting various tracers or pharmacologically active or inactive substances, including chemotherapeutics, cytokines, viral particles, and liposomes.

Abstract

Convection-enhanced delivery (CED) is a neurosurgical technique enabling effective perfusion of large brain volumes using a catheter system. Such an approach provides a safe delivery method by-passing the blood brain barrier (BBB), thus allowing treatment with therapeutics with poor BBB-permeability or those for which systemic exposure is not desired, e.g., due to toxicity. CED requires optimization of the catheter design, injection protocol, and properties of the infusate. With this protocol we describe how to perform CED of a solution containing up to 20 µg of an antibody into the caudate putamen of mice. It describes preparation of step catheters, testing them in vitro and performing the CED in mice using a ramping injection program. The protocol can be readily adjusted for other infusion volumes and can be used for injecting various tracers or pharmacologically active or inactive substances, including chemotherapeutics, cytokines, viral particles, and liposomes.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Laboratory Animal Science
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:General Biochemistry, Genetics and Molecular Biology, General Immunology and Microbiology, General Chemical Engineering, General Neuroscience
Language:English
Date:18 July 2019
Deposited On:28 Jan 2020 16:21
Last Modified:29 Jan 2020 08:36
Publisher:Journal of Visualized Experiments
ISSN:1940-087X
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.3791/59675
Official URL:https://www.jove.com/video/59675
Project Information:
  • : FunderUniversity of Zurich
  • : Grant IDFK-15-057
  • : Project Title
  • : FunderNovartis Foundation for medical-biological Research
  • : Grant ID16C231
  • : Project Title
  • : FunderSwiss Cancer Research
  • : Grant IDKFS-3852-02-2016, KFS-4146-02-2017
  • : Project Title
  • : FunderBRIDGE Proof of Concept
  • : Grant ID20B1-1_177300
  • : Project Title

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Content: Published Version
Language: English
Filetype: PDF - Registered users only until 18 July 2021
Size: 358kB
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Embargo till: 2021-07-18