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Chaperonin overproduction and metabolic erosion caused by mutation accumulation in Escherichia coli


Aguilar-Rodríguez, José; Fares, Mario A; Wagner, Andreas (2019). Chaperonin overproduction and metabolic erosion caused by mutation accumulation in Escherichia coli. FEMS Microbiology Letters, 366(10):fnz121.

Abstract

Bacterial cells adapting to a constant environment tend to accumulate mutations in portions of their genome that are not maintained by selection. This process has been observed in bacteria evolving under strong genetic drift, and especially in bacterial endosymbionts of insects. Here, we study this process in hypermutable Escherichia coli populations evolved through 250 single-cell bottlenecks on solid rich medium in a mutation accumulation experiment that emulates the evolution of bacterial endosymbionts. Using phenotype microarrays monitoring metabolic activity in 95 environments distinguished by their carbon sources, we observe how mutation accumulation has decreased the ability of cells to metabolize most carbon sources. We study if the chaperonin GroEL, which is naturally overproduced in bacterial endosymbionts, can ameliorate the process of metabolic erosion, because of its known ability to buffer destabilizing mutations in metabolic enzymes. Our results indicate that GroEL can slow down the negative phenotypic consequences of genome decay in some environments.

Abstract

Bacterial cells adapting to a constant environment tend to accumulate mutations in portions of their genome that are not maintained by selection. This process has been observed in bacteria evolving under strong genetic drift, and especially in bacterial endosymbionts of insects. Here, we study this process in hypermutable Escherichia coli populations evolved through 250 single-cell bottlenecks on solid rich medium in a mutation accumulation experiment that emulates the evolution of bacterial endosymbionts. Using phenotype microarrays monitoring metabolic activity in 95 environments distinguished by their carbon sources, we observe how mutation accumulation has decreased the ability of cells to metabolize most carbon sources. We study if the chaperonin GroEL, which is naturally overproduced in bacterial endosymbionts, can ameliorate the process of metabolic erosion, because of its known ability to buffer destabilizing mutations in metabolic enzymes. Our results indicate that GroEL can slow down the negative phenotypic consequences of genome decay in some environments.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:30 May 2019
Deposited On:07 Feb 2020 08:48
Last Modified:11 Feb 2020 09:49
Publisher:Oxford University Press
ISSN:0378-1097
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/femsle/fnz121
Project Information:
  • : FunderSNSF
  • : Grant IDP2ZHP3_174735
  • : Project TitleGenotype-fitness map of mutations in the Ras/PKA and TOR/Sch9 signaling pathways in the budding yeast Saccharomyces cerevisiae
  • : FunderSNSF
  • : Grant ID31003A_172887
  • : Project TitleRobustness and weakened selection in the adaptive evolution of fluorescent proteins
  • : FunderH2020
  • : Grant ID739874
  • : Project TitleNoiseRobustEvo - Noise and robustness in the evolution of novel protein phenotypes

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