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Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironments

Blache, Ulrich; Horton, Edward R; Xia, Tian; Schoof, Erwin M; Blicher, Lene H; Schönenberger, Angelina; Snedeker, Jess G; Martin, Ivan; Erler, Janine T; Ehrbar, Martin (2019). Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironments. Life Science Alliance, 2(3):e201900304.

Abstract

Mesenchymal stromal cells (MSCs) are key contributors of the tumour microenvironment and are known to promote cancer progression through reciprocal communication with cancer cells, but how they become activated is not fully understood. Here, we investigate how breast cancer cells from different stages of the metastatic cascade convert MSCs into tumour-associated MSCs (TA-MSCs) using unbiased, global approaches. Using mass spectrometry, we compared the secretomes of MCF-7 cells, invasive MDA-MB-231 cells, and sublines isolated from bone, lung, and brain metastases and identified ECM and exosome components associated with invasion and organ-specific metastasis. Next, we used synthetic hydrogels to investigate how these different secretomes activate MSCs in bioengineered 3D microenvironments. Using kinase activity profiling and RNA sequencing, we found that only MDA-MB-231 breast cancer secretomes convert MSCs into TA-MSCs, resulting in an immunomodulatory phenotype that was particularly prominent in response to bone-tropic cancer cells. We have investigated paracrine signalling from breast cancer cells to TA-MSCs in 3D, which may highlight new potential targets for anticancer therapy approaches aimed at targeting tumour stroma.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
04 Faculty of Medicine > Balgrist University Hospital, Swiss Spinal Cord Injury Center
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Physical Sciences > Ecology
Life Sciences > Biochemistry, Genetics and Molecular Biology (miscellaneous)
Life Sciences > Plant Science
Physical Sciences > Health, Toxicology and Mutagenesis
Language:English
Date:June 2019
Deposited On:07 Feb 2020 16:23
Last Modified:21 Jun 2025 02:10
Publisher:Life Science Alliance
ISSN:2575-1077
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.26508/lsa.201900304
PubMed ID:31160380
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  • Content: Published Version
  • Language: English
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)

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