Header

UZH-Logo

Maintenance Infos

Seasonality of the antibiotic resistance gene blaCTX-M in temperate Lake Maggiore


Eckert, Ester M; Di Cesare, Andrea; Malki, Lala-Sakina; Villiger, Jörg; Pernthaler, Jakob; Callieri, Cristiana; Bertoni, Roberto; Corno, Gianluca (2019). Seasonality of the antibiotic resistance gene blaCTX-M in temperate Lake Maggiore. Hydrobiologia, 843(1):143-153.

Abstract

The beta lactamase gene blaCTX-M, responsible of the resistance to cephalosporins, has been detected in microbes from hospitals to open waters. We studied the seasonality and stability of blaCTX-M in Lake Maggiore over 3 years and the role of potential inputs of allochthonous bacteria and/or antibiotic pollution in promoting its occurrence. blaCTX-M was mainly present from January to July in the pelagic microbial community and the gene occurrence was significantly related to low water temperature. To evaluate its temporal stability in the bacterial community over a short period, we measured blaCTX-M daily over the course of 6 days. The gene was below the limit of quantification except for one sampling when its abundance peaked, suggesting a point contamination. The bacterial community of the lake in which blaCTX-M was detected suggests that at least two distinct bacterial populations contained the gene. The occurrence of known blaCTX-M containing genera and the occurrence of the gene, however, did not overlap. Furthermore, the experimental addition of cefotaxime to lake water incubations did not promote abundance of the gene. These data imply that blaCTX-M was present in the environmental microbial community. Increases of gene abundances were likely caused by environmental parameters other than antibiotic contamination.

Abstract

The beta lactamase gene blaCTX-M, responsible of the resistance to cephalosporins, has been detected in microbes from hospitals to open waters. We studied the seasonality and stability of blaCTX-M in Lake Maggiore over 3 years and the role of potential inputs of allochthonous bacteria and/or antibiotic pollution in promoting its occurrence. blaCTX-M was mainly present from January to July in the pelagic microbial community and the gene occurrence was significantly related to low water temperature. To evaluate its temporal stability in the bacterial community over a short period, we measured blaCTX-M daily over the course of 6 days. The gene was below the limit of quantification except for one sampling when its abundance peaked, suggesting a point contamination. The bacterial community of the lake in which blaCTX-M was detected suggests that at least two distinct bacterial populations contained the gene. The occurrence of known blaCTX-M containing genera and the occurrence of the gene, however, did not overlap. Furthermore, the experimental addition of cefotaxime to lake water incubations did not promote abundance of the gene. These data imply that blaCTX-M was present in the environmental microbial community. Increases of gene abundances were likely caused by environmental parameters other than antibiotic contamination.

Statistics

Citations

Dimensions.ai Metrics
1 citation in Web of Science®
1 citation in Scopus®
Google Scholar™

Altmetrics

Downloads

8 downloads since deposited on 07 Feb 2020
7 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Scopus Subject Areas:Life Sciences > Aquatic Science
Uncontrolled Keywords:Aquatic Science, Pollution, General Environmental Science
Language:English
Date:1 October 2019
Deposited On:07 Feb 2020 15:53
Last Modified:10 Aug 2020 00:00
Publisher:Springer
ISSN:0018-8158
OA Status:Green
Publisher DOI:https://doi.org/10.1007/s10750-019-04043-w

Download

Green Open Access

Download PDF  'Seasonality of the antibiotic resistance gene blaCTX-M in temperate Lake Maggiore'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 384kB
View at publisher