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A simple guide for generating BAC transgenic animals for retinal research


Agca, Cavit; Grimm, Christian (2020). A simple guide for generating BAC transgenic animals for retinal research. Methods in Molecular Biology, 2092:109-122.

Abstract

Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.

Abstract

Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Ophthalmology Clinic
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Uncontrolled Keywords:BAC sequencing; BAC transgenics; Colony real-time PCR; Electroporation; Lif; Minimal media; Recombineering; SW102; galK
Language:English
Date:2020
Deposited On:21 Feb 2020 15:01
Last Modified:29 Jul 2020 14:14
Publisher:Springer
ISSN:1064-3745
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-0716-0175-4_9
PubMed ID:31786785

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