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Atypical hemolytic Listeria innocua isolates are virulent, albeit less than Listeria monocytogenes


Moura, Alexandra; Disson, Olivier; Lavina, Morgane; Thouvenot, Pierre; Huang, Lei; Leclercq, Alexandre; Fredriksson-Ahomaa, Maria; Eshwar, Athmanya K; Stephan, Roger; Lecuit, Marc (2019). Atypical hemolytic Listeria innocua isolates are virulent, albeit less than Listeria monocytogenes. Infection and Immunity, 87(4):e00758-18.

Abstract

<jats:named-content content-type="genus-species">Listeria innocua</jats:named-content> is considered a nonpathogenic <jats:italic>Listeria</jats:italic> species. Natural atypical hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> isolates have been reported but have not been characterized in detail. Here, we report the genomic and functional characterization of representative isolates from the two known natural hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> clades. Whole-genome sequencing confirmed the presence of <jats:italic>Listeria</jats:italic> pathogenicity islands (LIPI) characteristic of <jats:named-content content-type="genus-species">Listeria monocytogenes</jats:named-content> species. Functional assays showed that LIPI-1 and <jats:italic>inlA</jats:italic> genes are transcribed, and the corresponding gene products are expressed and functional. Using <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> assays, we show that atypical hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> is virulent, can actively cross the intestinal epithelium, and spreads systemically to the liver and spleen, albeit to a lesser degree than the reference <jats:named-content content-type="genus-species">L. monocytogenes</jats:named-content> EGDe strain. Although human exposure to hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> is likely rare, these findings are important for food safety and public health. The presence of virulence traits in some <jats:named-content content-type="genus-species">L. innocua</jats:named-content> clades supports the existence of a common virulent ancestor of <jats:named-content content-type="genus-species">L. monocytogenes</jats:named-content> and <jats:named-content content-type="genus-species">L. innocua</jats:named-content>.

Abstract

<jats:named-content content-type="genus-species">Listeria innocua</jats:named-content> is considered a nonpathogenic <jats:italic>Listeria</jats:italic> species. Natural atypical hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> isolates have been reported but have not been characterized in detail. Here, we report the genomic and functional characterization of representative isolates from the two known natural hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> clades. Whole-genome sequencing confirmed the presence of <jats:italic>Listeria</jats:italic> pathogenicity islands (LIPI) characteristic of <jats:named-content content-type="genus-species">Listeria monocytogenes</jats:named-content> species. Functional assays showed that LIPI-1 and <jats:italic>inlA</jats:italic> genes are transcribed, and the corresponding gene products are expressed and functional. Using <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> assays, we show that atypical hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> is virulent, can actively cross the intestinal epithelium, and spreads systemically to the liver and spleen, albeit to a lesser degree than the reference <jats:named-content content-type="genus-species">L. monocytogenes</jats:named-content> EGDe strain. Although human exposure to hemolytic <jats:named-content content-type="genus-species">L. innocua</jats:named-content> is likely rare, these findings are important for food safety and public health. The presence of virulence traits in some <jats:named-content content-type="genus-species">L. innocua</jats:named-content> clades supports the existence of a common virulent ancestor of <jats:named-content content-type="genus-species">L. monocytogenes</jats:named-content> and <jats:named-content content-type="genus-species">L. innocua</jats:named-content>.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Immunology, Microbiology, Parasitology, Infectious Diseases, L. innocua ; LIPI; inlA ; listeriosis; virulence
Language:English
Date:22 January 2019
Deposited On:14 Feb 2020 16:20
Last Modified:14 Feb 2020 16:20
Publisher:American Society for Microbiology
ISSN:0019-9567
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1128/iai.00758-18
PubMed ID:30670551

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