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Phenotypic and genotypic characterization of clinical isolates belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex isolated from animals treated at a Veterinary Hospital in Switzerland


Püntener-Simmen, Sabrina; Zurfluh, Katrin; Schmitt, Sarah; Stephan, Roger; Nüesch-Inderbinen, Magdalena (2019). Phenotypic and genotypic characterization of clinical isolates belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex isolated from animals treated at a Veterinary Hospital in Switzerland. Frontiers in Veterinary Science, 6:17.

Abstract

Objectives: We investigated a collection of strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex obtained from a veterinary clinic with regard to their genetic relatedness, presence of antibiotic resistance genes and antimicrobial susceptibility profiles. Methods: Fifty-eight ACB-complex strains from animals treated at a veterinary clinic between 2006 and 2017, and seven strains collected from the hospital environment during 2012 were analyzed. Assignment to sequence types (ST) and international complexes (IC) was done by multilocus sequence typing (MLST) according to the Pasteur scheme. Genes encoding carbapenemases, aminoglycoside-modifying enzymes, macrolide-, quinolone- and co-trimoxazole resistance genes, the ISAba1 element, virulence associated intI1 genes and plasmid associated toxin-antitoxin markers were identified by microarray. Genes encoding bla OXA-51-like carbapenemases were amplified by PCR and sequenced. Susceptibility profiles were determined by disc diffusion or by broth microdilution. Results: Among 50 A. baumannii isolates from animals, two predominant clones were observed linked to CC1 (n = 27/54% of the isolates) and CC25 (n = 14/28%), respectively. Strains of IC I harbored bla OXA-69, aac(3')-la, aadA1, sul1, intI1, and splA/T genes. Isolates belonging to CC25 possessed bla OXA-64. Six (12%) isolates belonging to CC2 and carrying bla OXA-66 were also noted. One isolate belonged to CC10 (bla OXA-68), one to CC149 (bla OXA-104), the remaining isolate was assigned to ST1220 and possessed bla OXA-116. Of six environmental A. baumannii, four (66.7%) belonged to CC25 (bla OXA-64), one (16.7%) to CC2 (bla OXA-66) and one to CC3 (bla OXA-71). Nine isolates (eight from animals and one environmental strain) were non-baumannii strains and did not harbor bla OXA-51-like genes. None of the isolates carried bla OXA-23, bla OXA-48, or bla OXA-58, and none were resistant to carbapenems. Conclusions: Clonal lineages of the veterinary A. baumannii isolates in our collection are identical to those globally emerging in humans but do not harbor bla OXA-23. A. baumannii CC25 may be specific for this particular veterinary clinic environment.

Abstract

Objectives: We investigated a collection of strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex obtained from a veterinary clinic with regard to their genetic relatedness, presence of antibiotic resistance genes and antimicrobial susceptibility profiles. Methods: Fifty-eight ACB-complex strains from animals treated at a veterinary clinic between 2006 and 2017, and seven strains collected from the hospital environment during 2012 were analyzed. Assignment to sequence types (ST) and international complexes (IC) was done by multilocus sequence typing (MLST) according to the Pasteur scheme. Genes encoding carbapenemases, aminoglycoside-modifying enzymes, macrolide-, quinolone- and co-trimoxazole resistance genes, the ISAba1 element, virulence associated intI1 genes and plasmid associated toxin-antitoxin markers were identified by microarray. Genes encoding bla OXA-51-like carbapenemases were amplified by PCR and sequenced. Susceptibility profiles were determined by disc diffusion or by broth microdilution. Results: Among 50 A. baumannii isolates from animals, two predominant clones were observed linked to CC1 (n = 27/54% of the isolates) and CC25 (n = 14/28%), respectively. Strains of IC I harbored bla OXA-69, aac(3')-la, aadA1, sul1, intI1, and splA/T genes. Isolates belonging to CC25 possessed bla OXA-64. Six (12%) isolates belonging to CC2 and carrying bla OXA-66 were also noted. One isolate belonged to CC10 (bla OXA-68), one to CC149 (bla OXA-104), the remaining isolate was assigned to ST1220 and possessed bla OXA-116. Of six environmental A. baumannii, four (66.7%) belonged to CC25 (bla OXA-64), one (16.7%) to CC2 (bla OXA-66) and one to CC3 (bla OXA-71). Nine isolates (eight from animals and one environmental strain) were non-baumannii strains and did not harbor bla OXA-51-like genes. None of the isolates carried bla OXA-23, bla OXA-48, or bla OXA-58, and none were resistant to carbapenems. Conclusions: Clonal lineages of the veterinary A. baumannii isolates in our collection are identical to those globally emerging in humans but do not harbor bla OXA-23. A. baumannii CC25 may be specific for this particular veterinary clinic environment.

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Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Acinetobacter; animals; antimicrobial resistance; blaOXA−51-like; genotypes
Language:English
Date:5 February 2019
Deposited On:14 Feb 2020 16:41
Last Modified:11 Mar 2020 16:01
Publisher:Frontiers Research Foundation
ISSN:2297-1769
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.3389/fvets.2019.00017
PubMed ID:30805352

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