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Stereological analysis of hippocampus in rat treated with chemotherapeutic agent oxaliplatin


Sadeghinezhad, J; Amrein, I (2020). Stereological analysis of hippocampus in rat treated with chemotherapeutic agent oxaliplatin. Folia Morphologica:Epub ahead of print.

Abstract

Background:
Oxaliplatin (OX) has been widely used for treatment of colorectal and other cancers. Adverse effect of OX and other anticancer agents on cognition have been reported, but studies on the effects of chemotherapy on brain structure are scarce. This study describes the morphometrical features of the hippocampus structures in rat following OX treatment using design-based stereological methods.

Materials and methods:
Ten male Wistar rats were randomized into two groups. The rats from OX group received 2.4 mg/kg OX in vehicle for five consecutive days every week for 2 weeks intraperitoneally (IP). Controls received vehicle only. Cavalieri 's method and the optical fractionator method were used for volume and neuron estimation, respectively.

Results:
Cavalieri 's method was used for to estimate volume and showed that the volume of the hippocampus was significantly decreased in OX group (31.84 ± 1.24 mm3) compared with the vehicle control group (36.95 ± 3.48 mm3). The optical fractionator method was used to estimate neuron number and showed that the number of neurons in dentate gyrus, cornu ammonis 1 and 3 in OX group (8.147 ± 2.84 × 105, 4.257 ± 0.59 × 105 and 2.133 ± 0.22 × 105, respectively) did not differ from those of vehicle control group (7.36 ± 1.42 × 105, 3.521 ± 0.54 × 105 and 1.989 ± 0.46 × 105, respectively).

Conclusions:
These findings suggested that OX treatment induce loss of hippocampal volume without neuronal loss which might help to clarify the mechanism by which OX affects cognition and to improve preventive treatment strategies.

Abstract

Background:
Oxaliplatin (OX) has been widely used for treatment of colorectal and other cancers. Adverse effect of OX and other anticancer agents on cognition have been reported, but studies on the effects of chemotherapy on brain structure are scarce. This study describes the morphometrical features of the hippocampus structures in rat following OX treatment using design-based stereological methods.

Materials and methods:
Ten male Wistar rats were randomized into two groups. The rats from OX group received 2.4 mg/kg OX in vehicle for five consecutive days every week for 2 weeks intraperitoneally (IP). Controls received vehicle only. Cavalieri 's method and the optical fractionator method were used for volume and neuron estimation, respectively.

Results:
Cavalieri 's method was used for to estimate volume and showed that the volume of the hippocampus was significantly decreased in OX group (31.84 ± 1.24 mm3) compared with the vehicle control group (36.95 ± 3.48 mm3). The optical fractionator method was used to estimate neuron number and showed that the number of neurons in dentate gyrus, cornu ammonis 1 and 3 in OX group (8.147 ± 2.84 × 105, 4.257 ± 0.59 × 105 and 2.133 ± 0.22 × 105, respectively) did not differ from those of vehicle control group (7.36 ± 1.42 × 105, 3.521 ± 0.54 × 105 and 1.989 ± 0.46 × 105, respectively).

Conclusions:
These findings suggested that OX treatment induce loss of hippocampal volume without neuronal loss which might help to clarify the mechanism by which OX affects cognition and to improve preventive treatment strategies.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Anatomy
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Anatomy, Histology
Language:English
Date:11 March 2020
Deposited On:07 Apr 2020 16:14
Last Modified:07 Apr 2020 16:14
Publisher:Wydawnictwo Via Medica
ISSN:0015-5659
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.5603/fm.a2020.0031

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