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PhotoTag: Photoactivatable Fluorophores for Protein Labeling


Fay, Rachael; Linden, Anthony; Holland, Jason P (2020). PhotoTag: Photoactivatable Fluorophores for Protein Labeling. Organic Letters, 22(9):3499-3503.

Abstract

We report experimental studies on the development of photoactivatable fluorophores for rapid, light-induced synthesis of protein conjugates. Proof-of-concept studies demonstrated that electronic excitation of photoactivatable BODIPY-ArN3 (1) in the presence of different proteins leads to efficient labeling in less than 10 min. After synthesis and isolation of the fluorescently tagged protein, photochemical conversion yields using human serum albumin and onartuzumab were 47 ± 7% and 42 ± 5%, respectively.

Abstract

We report experimental studies on the development of photoactivatable fluorophores for rapid, light-induced synthesis of protein conjugates. Proof-of-concept studies demonstrated that electronic excitation of photoactivatable BODIPY-ArN3 (1) in the presence of different proteins leads to efficient labeling in less than 10 min. After synthesis and isolation of the fluorescently tagged protein, photochemical conversion yields using human serum albumin and onartuzumab were 47 ± 7% and 42 ± 5%, respectively.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Life Sciences > Biochemistry
Physical Sciences > Physical and Theoretical Chemistry
Physical Sciences > Organic Chemistry
Uncontrolled Keywords:Physical and Theoretical Chemistry, Organic Chemistry, Biochemistry
Language:English
Date:1 May 2020
Deposited On:17 Apr 2020 09:35
Last Modified:29 Jul 2020 15:02
Publisher:American Chemical Society (ACS)
ISSN:1523-7052
OA Status:Closed
Publisher DOI:https://doi.org/10.1021/acs.orglett.0c00957
Project Information:
  • : FunderSNSF
  • : Grant IDPP00P2_163683
  • : Project TitleAdvanced radiochemical methods for multi-modal imaging with nanomedicines
  • : FunderH2020
  • : Grant ID676904
  • : Project TitleNanoSCAN - Developing multi-modality nanomedicines for targeted annotation of oncogenic signaling pathways
  • : FunderSNSF
  • : Grant IDPP00P2_190093
  • : Project Title
  • : FunderKrebsliga
  • : Grant IDKLS-4257-08-2017
  • : Project Title

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Language: English
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Embargo till: 2021-04-13