Header

UZH-Logo

Maintenance Infos

Secreted microRNA data from the parasitic filarial nematode Acanthocheilonema viteae


Soichot, Julien; Endriss, Yvette; Marti, Hanspeter; Tritten, Lucienne (2020). Secreted microRNA data from the parasitic filarial nematode Acanthocheilonema viteae. Data in Brief, 29:105334.

Abstract

microRNAs (miRNAs) are an abundant class of non-coding RNA species with important regulatory roles in gene expression at the posttranscriptional level. The helminth Acanthocheilonema viteae serves as model organism for research on parasitic filarial nematodes. Total RNA secreted or excreted in vitro by 1500 adult female and male A. viteae over 3 weeks was isolated from culture media previously processed by differential ultracentrifugation. miRNA sequencing revealed the presence of 360 unique miRNA candidates released by adult A. viteae in vitro. Among them, 74 high-confidence unique miRNAs, as well as several potential novel miRNA candidates were discovered. A large proportion of the sequenced miRNA candidates appeared differentially expressed between the male and female samples based on normalized copy count. The presence of extracellular vesicles, often rich in miRNAs, could not be confirmed unambiguously by transmission electron microscopy.

Abstract

microRNAs (miRNAs) are an abundant class of non-coding RNA species with important regulatory roles in gene expression at the posttranscriptional level. The helminth Acanthocheilonema viteae serves as model organism for research on parasitic filarial nematodes. Total RNA secreted or excreted in vitro by 1500 adult female and male A. viteae over 3 weeks was isolated from culture media previously processed by differential ultracentrifugation. miRNA sequencing revealed the presence of 360 unique miRNA candidates released by adult A. viteae in vitro. Among them, 74 high-confidence unique miRNAs, as well as several potential novel miRNA candidates were discovered. A large proportion of the sequenced miRNA candidates appeared differentially expressed between the male and female samples based on normalized copy count. The presence of extracellular vesicles, often rich in miRNAs, could not be confirmed unambiguously by transmission electron microscopy.

Statistics

Citations

Altmetrics

Downloads

1 download since deposited on 31 Aug 2020
1 download since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
600 Technology
Scopus Subject Areas:Health Sciences > Multidisciplinary
Uncontrolled Keywords:Multidisciplinary, Excretory-secretory; Extracellular vesicle; Filarial nematode; Parasite; microRNA.
Language:English
Date:April 2020
Deposited On:31 Aug 2020 15:42
Last Modified:04 Sep 2020 12:42
Publisher:Elsevier
ISSN:2352-3409
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.dib.2020.105334
PubMed ID:32181299
Project Information:
  • : FunderSNSF
  • : Grant IDPZ00P3_168080
  • : Project TitleSecretory microRNAs of nematode parasites: small molecules for great success?

Download

Gold Open Access

Download PDF  'Secreted microRNA data from the parasitic filarial nematode Acanthocheilonema viteae'.
Preview
Content: Published Version
Language: English
Filetype: PDF
Size: 180kB
View at publisher
Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)