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Simple targeted assays for metabolic pathways and signaling: a powerful tool for targeted proteomics


Abstract

We introduce STAMPS, a pathway centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ~150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin sensitive and resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

Abstract

We introduce STAMPS, a pathway centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ~150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin sensitive and resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Clinical Chemistry
Dewey Decimal Classification:610 Medicine & health
540 Chemistry
Language:English
Date:31 August 2020
Deposited On:18 Sep 2020 07:14
Last Modified:19 Feb 2021 09:28
Publisher:American Chemical Society (ACS)
ISSN:0003-2700
OA Status:Closed
Publisher DOI:https://doi.org/10.1021/acs.analchem.0c02793
PubMed ID:32865986

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