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Chlamydia suis is associated with intestinal NF-κB activation in experimentally infected gnotobiotic piglets


Aumayer, Helen; Leonard, Cory Ann; Pesch, Theresa; Prähauser, Barbara; Wunderlin, Sabina; Guscetti, Franco; Borel, Nicole (2020). Chlamydia suis is associated with intestinal NF-κB activation in experimentally infected gnotobiotic piglets. FEMS Pathogens and Disease, 78(6):ftaa040.

Abstract

Chlamydia suis intestinal infection of single-animal experimental groups of gnotobiotic newborn piglets was previously reported to cause severe, temporary small intestinal epithelium damage. We investigated archived intestinal samples for pro-inflammatory nuclear factor kappa B (NF-κB) activation, Interleukin (IL)-6 and IL-8 production and immune cell influx. Samples were collected 2, 4 and 7 days post-inoculation with C. suis strain S45/6 or mock inoculum (control). Increased nuclear localization of epithelial NF-κB, representative of activation, in the jejunum and ileum of C. suis-infected animals, compared to uninfected controls, began by 2 days post-infection (dpi) and persisted through 7 dpi. Infected animals showed increased production of IL-8, peaking at 2 dpi, compared to controls. Infection-mediated CD45-positive immune cell influx into the jejunal lamina propria peaked at 7 dpi, when epithelial damage was largely resolved. Activation of NF-κB appears to be a key early event in the innate response of the unprimed porcine immune system challenged with C. suis. This results in an acute phase, coinciding with the most severe clinical symptoms, diarrhea and weight loss. Immune cells recruited shortly after infection remain present in the lamina propria during the recovery phase, which is characterized by reduced chlamydial shedding and restored intestinal epithelium integrity.

Abstract

Chlamydia suis intestinal infection of single-animal experimental groups of gnotobiotic newborn piglets was previously reported to cause severe, temporary small intestinal epithelium damage. We investigated archived intestinal samples for pro-inflammatory nuclear factor kappa B (NF-κB) activation, Interleukin (IL)-6 and IL-8 production and immune cell influx. Samples were collected 2, 4 and 7 days post-inoculation with C. suis strain S45/6 or mock inoculum (control). Increased nuclear localization of epithelial NF-κB, representative of activation, in the jejunum and ileum of C. suis-infected animals, compared to uninfected controls, began by 2 days post-infection (dpi) and persisted through 7 dpi. Infected animals showed increased production of IL-8, peaking at 2 dpi, compared to controls. Infection-mediated CD45-positive immune cell influx into the jejunal lamina propria peaked at 7 dpi, when epithelial damage was largely resolved. Activation of NF-κB appears to be a key early event in the innate response of the unprimed porcine immune system challenged with C. suis. This results in an acute phase, coinciding with the most severe clinical symptoms, diarrhea and weight loss. Immune cells recruited shortly after infection remain present in the lamina propria during the recovery phase, which is characterized by reduced chlamydial shedding and restored intestinal epithelium integrity.

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Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Institute of Veterinary Pathology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Health Sciences > Immunology and Allergy
Life Sciences > General Immunology and Microbiology
Health Sciences > Microbiology (medical)
Health Sciences > Infectious Diseases
Uncontrolled Keywords:Immunology and Allergy, Microbiology (medical), General Immunology and Microbiology, Infectious Diseases, General Medicine
Language:English
Date:1 August 2020
Deposited On:24 Sep 2020 15:14
Last Modified:24 Jul 2024 01:35
Publisher:Oxford University Press
ISSN:2049-632X
Additional Information:This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Pathogens and disease following peer review. The definitive publisher-authenticated version Aumayer H, Leonard CA, Pesch T, Prähauser B, Wunderlin S, Guscetti F, Borel N. Chlamydia suis is associated with intestinal NF-κB activation in experimentally infected gnotobiotic piglets. Pathog Dis. 2020 Aug 1;78(6):ftaa040. doi: 10.1093/femspd/ftaa040. PMID: 32804203 is available online at: https://academic.oup.com/femspd/article/78/6/ftaa040/5893292
OA Status:Hybrid
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/femspd/ftaa040
PubMed ID:32804203
Project Information:
  • : FunderSNSF
  • : Grant ID310030_147026
  • : Project TitlePersistence mechanism in a mixed infection model with Chlamydia and porcine epidemic diarrhea virus