Header

UZH-Logo

Maintenance Infos

Deciphering the Enigmatic Function of Pseudomonas Metallothioneins


Habjanič, Jelena; Mathew, Anugraha; Eberl, Leo; Freisinger, Eva (2020). Deciphering the Enigmatic Function of Pseudomonas Metallothioneins. Frontiers in Microbiology, 11:1709.

Abstract

Metallothioneins (MTs) are low molecular weight, Cys-rich proteins that sequester both essential and non-essential metal ions. Despite being highly conserved in the Pseudomonas genus of Gram-negative bacteria, knowledge of their physiological function in this species is scarce. Using the strain P. fluorescens Q2-87 as a model organism, we investigated the role of a conserved MT in zinc homeostasis, cadmium detoxification as well as its implications in stress response. We show that MT expression is only induced in the stationary phase and provides a fitness benefit for long-term starvation survival, while it is not required for metal resistance and acquisition, oxidative or nitrosative stress response, biofilm formation or motility.

Abstract

Metallothioneins (MTs) are low molecular weight, Cys-rich proteins that sequester both essential and non-essential metal ions. Despite being highly conserved in the Pseudomonas genus of Gram-negative bacteria, knowledge of their physiological function in this species is scarce. Using the strain P. fluorescens Q2-87 as a model organism, we investigated the role of a conserved MT in zinc homeostasis, cadmium detoxification as well as its implications in stress response. We show that MT expression is only induced in the stationary phase and provides a fitness benefit for long-term starvation survival, while it is not required for metal resistance and acquisition, oxidative or nitrosative stress response, biofilm formation or motility.

Statistics

Citations

Dimensions.ai Metrics
10 citations in Web of Science®
11 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

19 downloads since deposited on 01 Oct 2020
5 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Life Sciences > Microbiology
Health Sciences > Microbiology (medical)
Uncontrolled Keywords:Microbiology (medical), Microbiology
Language:English
Date:22 July 2020
Deposited On:01 Oct 2020 09:12
Last Modified:27 Jan 2022 02:39
Publisher:Frontiers Research Foundation
ISSN:1664-302X
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.3389/fmicb.2020.01709
  • Content: Published Version
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)