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Quercetin reduces erosive dentin wear: Evidence from laboratory and clinical studies


Jiang, Neng-Wu; Hong, Deng-Wei; Attin, Thomas; Cheng, Hui; Yu, Hao (2020). Quercetin reduces erosive dentin wear: Evidence from laboratory and clinical studies. Dental Materials, 36(11):1430-1436.

Abstract

Objective

The aim of the present study was to evaluate the effect of quercetin on the acid resistance of human dentin through both laboratory and clinical studies.
Methods

Two hundred and twelve dentin blocks (2 mm × 2 mm × 2 mm) were prepared and used. For the laboratory study, dentin specimens were randomly divided into 8 groups (n = 12): deionized water, ethanol, 1.23 × 104 μg/ml sodium fluoride (NaF), 120 μg/ml chlorhexidine, 183.2 μg/ml epigallocatechin gallate (EGCG), and 75 μg/ml, 150 μg/ml, and 300 μg/ml quercetin (Q75, Q150, and Q300). The specimens were treated with the respective solutions for 2 min and then subjected to in vitro erosion (4 cycles/d for 7 d). The surface microhardness loss (%SMHl), erosive dentin wear, and surface morphology were evaluated and compared. For the impact on MMP inhibition, the release of crosslinked carboxyterminal telopeptide of type I collagen (ICTP) and the thickness of the demineralized organic matrix (DOM) were measured using additional dentin specimens. For the clinical study, the specimens were treated with NaF or Q300 for 2 min and then subjected to in vivo erosion (4 cycles/d for 7 d). The %SMHl and erosive dentin wear of the specimens were measured to determine whether quercetin similarly inhibits erosion in situ.
Results

The quercetin-treated group had a significantly lower %SMHl and erosive dentin wear than any other group, and the effect was concentration-dependent in vitro (P < 0.05). Dentin treated with quercetin produced significantly less ICTP and had a thicker DOM than the control dentin (P < 0.05). After in vivo erosion, the %SMHl and erosive dentin wear of the Q300 group were significantly lower than those of the control group (P < 0.05).
Significance

The application of quercetin was shown, for the first time, to increase the acid resistance of human dentin, possibly through MMP inhibition and DOM preservation.

Abstract

Objective

The aim of the present study was to evaluate the effect of quercetin on the acid resistance of human dentin through both laboratory and clinical studies.
Methods

Two hundred and twelve dentin blocks (2 mm × 2 mm × 2 mm) were prepared and used. For the laboratory study, dentin specimens were randomly divided into 8 groups (n = 12): deionized water, ethanol, 1.23 × 104 μg/ml sodium fluoride (NaF), 120 μg/ml chlorhexidine, 183.2 μg/ml epigallocatechin gallate (EGCG), and 75 μg/ml, 150 μg/ml, and 300 μg/ml quercetin (Q75, Q150, and Q300). The specimens were treated with the respective solutions for 2 min and then subjected to in vitro erosion (4 cycles/d for 7 d). The surface microhardness loss (%SMHl), erosive dentin wear, and surface morphology were evaluated and compared. For the impact on MMP inhibition, the release of crosslinked carboxyterminal telopeptide of type I collagen (ICTP) and the thickness of the demineralized organic matrix (DOM) were measured using additional dentin specimens. For the clinical study, the specimens were treated with NaF or Q300 for 2 min and then subjected to in vivo erosion (4 cycles/d for 7 d). The %SMHl and erosive dentin wear of the specimens were measured to determine whether quercetin similarly inhibits erosion in situ.
Results

The quercetin-treated group had a significantly lower %SMHl and erosive dentin wear than any other group, and the effect was concentration-dependent in vitro (P < 0.05). Dentin treated with quercetin produced significantly less ICTP and had a thicker DOM than the control dentin (P < 0.05). After in vivo erosion, the %SMHl and erosive dentin wear of the Q300 group were significantly lower than those of the control group (P < 0.05).
Significance

The application of quercetin was shown, for the first time, to increase the acid resistance of human dentin, possibly through MMP inhibition and DOM preservation.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Physical Sciences > General Materials Science
Health Sciences > General Dentistry
Physical Sciences > Mechanics of Materials
Language:English
Date:11 September 2020
Deposited On:05 Jan 2021 17:39
Last Modified:24 Apr 2024 01:49
Publisher:Elsevier
ISSN:0109-5641
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.dental.2020.08.013
PubMed ID:32928560