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Erosive loss of tooth substance is dependent on enamel surface structure and presence of pellicle - An in vitro study


Steiger-Ronay, Valerie; Kuster, Irina M; Wiedemeier, Daniel B; Attin, Thomas; Wegehaupt, Florian J (2020). Erosive loss of tooth substance is dependent on enamel surface structure and presence of pellicle - An in vitro study. Archives of Oral Biology, 112:104686.

Abstract

OBJECTIVE
Aim of this in vitro study was to investigate erosive tooth loss in dependence of the enamel surface structure and presence of an acquired pellicle.
METHODS
Enamel specimens from 19 bovine incisors (4 specimens/incisor) were allocated to four experimental groups (n = 19). The surfaces of half of the specimens were polished (two groups), while the other half was left native (two groups). Specimens of one polished and one native group were placed in pooled human saliva (30 min) for the formation of an acquired pellicle. Thereafter, all specimens were demineralized by superfusion with hydrochloric acid (17 min, pH 2.3) with collection of the superfluent. Erosive substance loss was determined by measuring the dissolved calcium content using a colorimetric assay with Arsenazo III reagent. Differences in erosive substance loss were statistically analyzed with respect to enamel surface and pellicle. A linear mixed effects model was fitted to the data and pairwise differences between groups were evaluated (significance level α= 0.05).
RESULTS
Enamel surface structure (p < 0.001) and presence of pellicle (p = 0.01) had a significant effect on erosive substance loss. Polished surfaces with pellicle showed the lowest cumulative calcium release [nmol Ca/mm$^{2}$] (means ± standard deviation: 48+/-5), followed by polished specimens without (51+/-9) and native specimens with pellicle (54+/-10). No significant differences were found between these groups. Highest cumulative calcium release was found for native specimens without pellicle (61+/-9; p < 0.05).
CONCLUSIONS
Both enamel surface structure and the acquired pellicle are important determinants of the susceptibility to erosive tooth loss.

Abstract

OBJECTIVE
Aim of this in vitro study was to investigate erosive tooth loss in dependence of the enamel surface structure and presence of an acquired pellicle.
METHODS
Enamel specimens from 19 bovine incisors (4 specimens/incisor) were allocated to four experimental groups (n = 19). The surfaces of half of the specimens were polished (two groups), while the other half was left native (two groups). Specimens of one polished and one native group were placed in pooled human saliva (30 min) for the formation of an acquired pellicle. Thereafter, all specimens were demineralized by superfusion with hydrochloric acid (17 min, pH 2.3) with collection of the superfluent. Erosive substance loss was determined by measuring the dissolved calcium content using a colorimetric assay with Arsenazo III reagent. Differences in erosive substance loss were statistically analyzed with respect to enamel surface and pellicle. A linear mixed effects model was fitted to the data and pairwise differences between groups were evaluated (significance level α= 0.05).
RESULTS
Enamel surface structure (p < 0.001) and presence of pellicle (p = 0.01) had a significant effect on erosive substance loss. Polished surfaces with pellicle showed the lowest cumulative calcium release [nmol Ca/mm$^{2}$] (means ± standard deviation: 48+/-5), followed by polished specimens without (51+/-9) and native specimens with pellicle (54+/-10). No significant differences were found between these groups. Highest cumulative calcium release was found for native specimens without pellicle (61+/-9; p < 0.05).
CONCLUSIONS
Both enamel surface structure and the acquired pellicle are important determinants of the susceptibility to erosive tooth loss.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Health Sciences > Otorhinolaryngology
Health Sciences > General Dentistry
Life Sciences > Cell Biology
Language:English
Date:April 2020
Deposited On:07 Jan 2021 09:08
Last Modified:08 Jan 2021 21:01
Publisher:Elsevier
ISSN:0003-9969
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.archoralbio.2020.104686
PubMed ID:32112967

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Language: English
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Embargo till: 2021-04-31