This study investigated the impact of a separate silanization step on the repair bond strength of composite substrates using a universal adhesive after various mechanical surface treatments. Composite specimens were aged and exposed to the following mechanical roughening treatments: diamond bur abrasion, aluminum oxide sandblasting, or silica coating. The specimens were then either left untreated or conditioned with universal adhesive (Scotchbond Universal), or a silane coupling agent was applied before the use of the universal adhesive or a conventional adhesive (Optibond FL). The conditioned surfaces and fresh substrate (positive control group) were covered with repair composite, and microtensile bond strength testing was performed. The significantly highest bond strength was obtained in the positive control group. Repair bond strength of the universal adhesive applied after a separate silanization step was similar to that without prior silanization, independent of the mechanical surface treatment. Moreover, after silica coating, no significant differences in the repair bond strength were detected among the different adhesive treatments. In conclusion, a separate silanization step before surface conditioning with the universal adhesive does not enhance the repair bond strength. On silica-coated composite substrates, repair bond strength values of the universal adhesive were similar to those of the conventional adhesive.